Mouse CD163 enzyme-linked immunoassay kit

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Catalog# EM0087

Mouse CD163 enzyme-linked immunoassay kit

Application

sELISA

Reactivity

Human

Standard curve range

156.25 ~10000 pg/ml

製品概要

製品名

Mouse CD163 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

15.62pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0521	0.0460	0.0491
156.25	0.1215	0.1155	0.1185	0.0695
312.50	0.2089	0.1872	0.1981	0.1490
625.00	0.2914	0.3162	0.3038	0.2548
1250.00	0.5026	0.5287	0.5157	0.4666
2500.00	0.8955	1.0390	0.9673	0.9182
5000.00	1.5630	1.6870	1.6250	1.5760
10000.00	2.6230	2.8050	2.7140	2.6650

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	pg/ml	O.D.	Average	Corrected	0.00	0.0521
Average (pg/ml)	0.0460	0.0491	156.25	0.1215	0.1155	0.1185
Standard Deviation	0.0695	312.50	0.2089	0.1872	0.1981	0.1490
Coefficient of Variation (%)	625.00	0.2914	0.3162	0.3038	0.2548	1250.00

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of mouse CD163 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 95% to 120% with an overall mean recovery of 109%.

サンプル値

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	185.36-256.77	100.0	229.54

Serum/Plasma– Thirty samples from apparently healthy mice were evaluated for the presence of mouse CD163 in this assay. No medical histories were available for the donors.n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: Q2VLH6 Mouse

別名

CD 163

CD163

CD163 antigen

CD163 molecule

Hemoglobin scavenger receptor

M130

M130 antigen precursor

Macrophage associated antigen

MM130

OTTHUMP00000238617

展開

機能

CD163, also known as M130 and p155, is a transmembrane scavenger receptor that is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10. A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.

Mouse CD163 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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