Human OX40/TNFRSF4 enzyme-linked immunoassay kit

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Catalog# EH0120

Human OX40/TNFRSF4 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

12.29~3000 pg/ml

製品概要

製品名

Human OX40/TNFRSF4 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

1.15 pg/ml (50 μl)；8.63 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0168	0.0150	0.0159
12.29	0.0413	0.0360	0.0387	0.0228
30.72	0.0754	0.0717	0.0736	0.0577
76.80	0.1554	0.1469	0.1512	0.1353
192.00	0.3511	0.3457	0.3484	0.3325
480.00	0.8416	0.8055	0.8236	0.8077
1200.00	1.8010	1.8460	1.8235	1.8076
3000.00	3.1860	3.2240	3.2050	3.1891

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	68.3	374.5	1141.6	69.2	348.2	1106.9
Standard Deviation	2.1	23.5	44.4	3.9	12.7	52.2
Coefficient of Variation (%)	3.0	6.3	3.9	5.6	3.7	4.7

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human OX40/TNFRSF4 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 120% with an overall mean recovery of 103%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	19.5-94.28	100	59.08

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of OX40/TNFRSF4 in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P43489 Human

別名

ACT 35

ACT35

ACT35 antigen

ATC35 antigen

CD 134

CD134

CD134 antigen

IMD16

Lymphoid activation antigene ACT35

OX 40

展開

機能

OX40 (CD134; TNFRSF4) is a T cell costimulatory molecule of the TNF receptor superfamily that coordinates with other membrane-bound costimulators such as CD28, CD40, CD30, CD27 and 4-1BB. OX40 is expressed on naïve CD4+ T cells only after engagement of the TCR by antigen presenting cells (APC; dendritic and B cells), and costimulation by CD40/CD40 ligand and CD28/B7. It is maximal at 2‑5 days post activation, or 4 hours post reactivation of memory T cells. Human OX40 is a 48 kDa type I transmembrane glycoprotein with a 28 amino acid (aa) signal sequence, a 185 aa extracellular domain (ECD) that has four TNFR-Cys repeats and an O-glycosylated hinge region, a 20 aa transmembrane segment, and a 41 aa cytoplasmic domain. The ECD of human OX40 shows 71%, 68%, 67%, 64% and 64% aa identity with feline, canine, rabbit, mouse and rat OX40 ECD, respectively. Engagement of OX40 on activated CD4+ T cells by OX40 ligand on activated dendritic cells promotes T cell survival and proliferation, prolongs the immune response, and enhances the number of cells making the transition from effector to memory T cells. OX40 signal transduction includes binding TNF receptor-associated factors (TRAFs), and activating NF kappa B and PI3 kinase to enhance expression of cytokines, antiapoptotic Bcl-2 family members, survivin and the chemokine receptor CXCR5. CXCR5 promotes T cell migration to germinal centers to deliver B cell help. Studies using knockout or transgenic mice, and agonistic or blocking antibodies, show that OX40/OX40L interaction is critical for establishing or reactivating memory T cells and breaking immune tolerance. Blockade of OX40 engagement is efficacious in animal models of allergic airway inflammation, graft-versus-host disease and autoimmune disease.

Human OX40/TNFRSF4 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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