Human CD31/PECAM-1 enzyme-linked immunoassay kit

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Catalog# EH0192

Human CD31/PECAM-1 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

6.86~5000 pg/ml

製品概要

製品名

Human CD31/PECAM-1 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.06 pg/ml (50 μl)；1.94 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0081	0.0084	0.0083
6.86	0.0211	0.0212	0.0212	0.0129
20.58	0.0481	0.0432	0.0457	0.0374
61.73	0.1258	0.1117	0.1188	0.1105
185.19	0.3603	0.3432	0.3518	0.3435
555.56	0.9777	0.8702	0.9240	0.9157
1666.67	2.2370	2.1020	2.1695	2.1613
5000.00	3.6500	3.6030	3.6265	3.6183

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	31.8	155.8	497.5	26.7	140.6	449.5
Standard Deviation	2.0	10.6	32.4	1.9	10.0	28.1
Coefficient of Variation (%)	6.4	6.8	6.5	7	7.1	6.2

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human CD31/PECAM-1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 118% with an overall mean recovery of 98%.

サンプル値

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	13.76-23.75	100	16.93

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD31/PECAM-1 in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P16284 Human

別名

Adhesion molecule

CD31

CD31 antigen

CD31 EndoCAM

EndoCAM

FLJ34100

FLJ58394

GPIIA

GPIIA'

PECA1

展開

機能

PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily. Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation. The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules. Metalloproteinase-mediated ectodomain shedding occurs during apoptosis but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis. In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse. Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions. Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis. In platelets, PECAM-1 is necessary for limiting thrombus formation and promoting integrin-mediated clot retraction and platelet spreading, but mechanisms for these phenomena are unclear. PECAM-/- mice are deficient in chemokine-mediated chemotaxis .

Human CD31/PECAM-1 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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