Human CD163 enzyme-linked immunoassay kit
Catalog# EH0185
Human CD163 enzyme-linked immunoassay kit
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ELISA
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Human
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0.14~100 ng/ml
製品概要
製品名
Human CD163 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
1.70 pg/ml (50 μl)；7.17 pg/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0099 | 0.0093 | 0.0096 | |
| 0.14 | 0.0621 | 0.0619 | 0.0620 | 0.0524 |
| 0.41 | 0.1733 | 0.1701 | 0.1717 | 0.1621 |
| 1.23 | 0.4638 | 0.4590 | 0.4614 | 0.4518 |
| 3.70 | 1.3069 | 1.2344 | 1.2707 | 1.2611 |
| 11.11 | 2.7103 | 2.6940 | 2.7022 | 2.6926 |
| 33.33 | 3.9264 | 3.9572 | 3.9418 | 3.9322 |
| 100.00 | 4.2301 | 4.2246 | 4.2274 | 4.2178 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 2.2 | 10.7 | 33.5 | 2.1 | 9.9 | 47.0 |
| Standard Deviation | 0.1 | 0.5 | 1.9 | 0.1 | 0.4 | 2.7 |
| Coefficient of Variation (%) | 4.8 | 4.6 | 5.7 | 3.2 | 3.8 | 5.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human CD163 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 115% with an overall mean recovery of 100%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 175.83-829.63 | 100 | 452.37 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD163 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
別名
C163A_HUMAN
CD 163
CD163
CD163 antigen
CD163 molecule
Hemoglobin scavenger receptor
M130
M130 antigen precursor
Macrophage associated antigen
MM130
展開