Human CCL2/MCP-1 enzyme-linked immunoassay kit
Catalog# EH0010
Human CCL2/MCP-1 enzyme-linked immunoassay kit
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ELISA
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Human
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0.14~100 pg/ml
製品概要
製品名
Human CCL2/MCP-1 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.002 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0098 | 0.0091 | 0.0095 | |
| 0.14 | 0.0280 | 0.0270 | 0.0275 | 0.0181 |
| 0.41 | 0.0654 | 0.0621 | 0.0638 | 0.0543 |
| 1.23 | 0.1784 | 0.1717 | 0.1751 | 0.1656 |
| 3.70 | 0.4950 | 0.4552 | 0.4751 | 0.4657 |
| 11.11 | 1.2670 | 1.1900 | 1.2285 | 1.2191 |
| 33.33 | 2.4260 | 2.3230 | 2.3745 | 2.3651 |
| 100.00 | 3.1270 | 3.0720 | 3.0995 | 3.0901 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 1.1 | 5.2 | 15.4 | 0.9 | 5.1 | 15.2 |
| Standard Deviation | 0.1 | 0.3 | 0.9 | 8.4 | 24.1 | 60.7 |
| Coefficient of Variation (%) | 6.1 | 5.4 | 5.8 | 6.3 | 4.8 | 5.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human CCL2/MCP-1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 118% with an overall mean recovery of 103%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 65.77-880.07 | 100 | 371.62 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CCL2 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
C-C motif chemokine 2
CCL2
CCL2_HUMAN
Chemokine (C C motif) ligand 2
GDCF 2
GDCF-2
GDCF2
HC11
HSMCR30
JE
展開