Human Flt3 Ligand/FLT3LG enzyme-linked immunoassay kit

見積もりを依頼する

お問い合わせ

E-mail: [email protected]

TEL: 0571-88062880

代理店・販売店を探す

Catalog# EH0255

Human Flt3 Ligand/FLT3LG enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

1.37~1000 pg/ml

製品概要

製品名

Human Flt3 Ligand/FLT3LG enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.05 pg/ml (50 μl)；1.08 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0121	0.0112	0.0117
1.37	0.0186	0.0188	0.0187	0.0071
4.12	0.0339	0.0336	0.0338	0.0221
12.35	0.0776	0.0749	0.0763	0.0646
37.04	0.1919	0.1890	0.1905	0.1788
111.11	0.4592	0.5409	0.5001	0.4884
333.33	1.4100	1.4120	1.4110	1.3994
1000.00	3.1510	3.1270	3.1390	3.1274

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	373.36	110.37	22.99	318.3	88.0	19.0
Standard Deviation	25.47	6.27	1.25	10.6	6.6	1.3
Coefficient of Variation (%)	6.8	5.7	5.4	3.3	2.3	6.8

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human Flt3 Ligand/FLT3LG into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 119% with an overall mean recovery of 105%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	53.86-214.88	100	101.93

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P49771 Human

機能

Flt‑3 Ligand, also known as FLT3L, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Mature human Flt‑3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. Within the ECD, human Flt‑3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt‑3 Ligand, respectively. The human and mouse Flt‑3 Ligand proteins show cross-species activity. Flt-3 Ligand is also structurally related to M-CSF and SCF. Flt-3 Ligand is widely expressed in various human and mouse tissues. It is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain of the Flt-3 Ligand dimer carries approximately 12 kDa of N- and O-linked carbohydrates. Alternate splicing and proteolytic cleavage of the transmembrane form of the Flt-3 Ligand protein can generate a soluble 30 kDa fragment that includes the cytokine-like domain. Alternate splicing of human Flt‑3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine-like domain in the ECD of the Flt-3 Ligand protein. Both transmembrane and soluble forms of Flt‑3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt‑3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. Additionally, Flt-3 Ligand synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. Flt-3 Ligand also cooperates with IL-2, IL-6, IL-7, and IL-15 to induce NK cell development and with IL-3, IL-7, and IL-11 to induce terminal B cell maturation. Animal studies show that Flt‑3 Ligand reduces the severity of experimentally induced allergic inflammation .

Human Flt3 Ligand/FLT3LG enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

機能

同一ターゲット・同一パスウェイ製品

Human/Monkey/Canine IL-12B/IL-12 p40 enzyme-linked immunoassay kit

Human CRP enzyme-linked immunoassay kit

Human Adiponectinenzyme-linked immunoassay kit（one step）

Human TNF RI/TNFRSF1A enzyme-linked immunoassay kit

Human Renin enzyme-linked immunoassay kit

Human/Monkey alpha Fetoprotein enzyme-linked immunoassay kit

Human von Willebrand Factor enzyme-linked immunoassay kit（one step）

Human Growth Hormone enzyme-linked immunoassay kit

Human Elafin/PI3 enzyme-linked immunoassay kit

Human IL-22 enzyme-linked immunoassay kit

Human Proprotein Convertase 9/PCSK9 enzyme-linked immunoassay kit

Human TRAIL/TNFSF10 enzyme-linked immunoassay kit

Human CCL13/MCP-4 enzyme-linked immunoassay kit

Human APOC3/Apolipoprotein CⅢ enzyme-linked immunoassay kit(one step)

Human CX3CL1/Fractalking enzyme-linked immunoassay kit

Human Neurogranin enzyme-linked immunoassay kit

Human CD80 enzyme-linked immunoassay kit

Human/Mouse MASP2 enzyme-linked immunoassay kit

Human Lipoprotein A/LPA enzyme-linked immunoassay kit

Human CD31/PECAM-1 enzyme-linked immunoassay kit

Human Angiotensinogen enzyme-linked immunoassay kit

Human EGFR enzyme-linked immunoassay kit

Human/Mouse GLP-1 Super Sensitivity enzyme-linked immunoassay kit

Human CD200 enzyme-linked immunoassay kit

Human FABP2 enzyme-linked immunoassay kit

Human S100A12 enzyme-linked immunoassay kit(one step)

Human VCAM-1/CD106 enzyme-linked immunoassay kit(one step)

Human Endostatin/COL18A1 enzyme-linked immunoassay kit(one step)

Human PAI-1/Serpin E1 enzyme-linked immunoassay kit(one step)

Human IL-6R enzyme-linked immunoassay kit(one step)

Human Leptin Receptor enzyme-linked immunoassay kit

Human/Monkey CCL11/Eotaxin enzyme-linked immunoassay kit

Human KIM-1/HAVCR1/TIM-1 enzyme-linked immunoassay kit

Human CD155/PVR(Poliovirus Receptor) enzyme-linked immunoassay kit(one step)

Human IL-17F enzyme-linked immunoassay kit

Human Apolipoprotein E/Apo E enzyme-linked immunoassay kit

Human CD115/M-CSF R/CSF-1-R enzyme-linked immunoassay kit（one step）

Human Siglec 9 enzyme-linked immunoassay kit

Human/Monkey Clusterin enzyme-linked immunoassay kit(one step)

Human CD27/TNFRSF7 enzyme-linked immunoassay kit

Human CTGF/CCN2 enzyme-linked immunoassay kit(one step)

Human GDF-15 enzyme-linked immunoassay kit

Human tPA enzyme-linked immunoassay kit(one step)

Human Prealbumin/Transthyretin enzyme-linked immunoassay kit(one step)

Human CCL2/MCP-1 enzyme-linked immunoassay kit

Human Acetylcholinesterase/ACHE enzyme-linked immunoassay kit

Human ICAM-1/CD54 enzyme-linked immunoassay kit（one step）

Human Apolipoprotein E/Apo E enzyme-linked immunoassay kit(one step)

Human/Monkey/Canine CCL5/RANTES enzyme-linked immunoassay kit(one step)

Human TREM2 enzyme-linked immunoassay kit（one step）

Human TGF-α enzyme-linked immunoassay kit

Human IL-2 enzyme-linked immunoassay kit

Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit

Human IL-15 enzyme-linked immunoassay kit

Human IFN-β enzyme-linked immunoassay kit

Human IL-7 enzyme-linked immunoassay kit

Human IL-33 enzyme-linked immunoassay kit