Human Myeloperoxidase(MPO) enzyme-linked immunoassay kit(one step)
Catalog# EHY0205
Human Myeloperoxidase(MPO) enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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0.14~100 ng/ml
製品概要
製品名
Human Myeloperoxidase(MPO) enzyme-linked immunoassay kit(one step)
製品タイプ
Elisa Kit
検出感度
0.02 ng/ml（50 μl）；0.07 ng/ml（10 μl）
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0088 | 0.0099 | 0.0094 | |
| 0.14 | 0.0226 | 0.0236 | 0.0231 | 0.0138 |
| 0.41 | 0.0502 | 0.0532 | 0.0517 | 0.0424 |
| 1.23 | 0.1425 | 0.1269 | 0.1347 | 0.1254 |
| 3.70 | 0.4044 | 0.4130 | 0.4087 | 0.3994 |
| 11.11 | 1.1640 | 1.1530 | 1.1585 | 1.1492 |
| 33.33 | 2.9250 | 3.0390 | 2.9820 | 2.9727 |
| 100.00 | 4.4116 | 4.5568 | 4.4842 | 4.4749 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.9 | 9.1 | 34.0 | 1.8 | 9.2 | 33.6 |
| Standard Deviation | 0.1 | 0.3 | 1.6 | 0.1 | 0.3 | 0.8 |
| Coefficient of Variation (%) | 3.1 | 3.7 | 4.7 | 4.7 | 2.9 | 2.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Myeloperoxidase into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 95% to 117% with an overall mean recovery of 109%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 3.72-19.70 | 100 | 7.8 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Myeloperoxidase in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
84 kDa myeloperoxidase
89 kDa myeloperoxidase
EC 1.11.1.7
EC1.11.2.2
fj80f04
MPO
mpx
myeloid-specific peroxidase
Myeloperoxidase
Myeloperoxidase heavy chain
展開