Human uPAR enzyme-linked immunoassay kit
Catalog# EH0077
Human uPAR enzyme-linked immunoassay kit
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ELISA
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Human
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27.43~20000 pg/ml
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unconjugated
製品概要
製品名
Human uPAR enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
8.79 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0148 | 0.0166 | 0.0157 | |
| 27.43 | 0.0262 | 0.0238 | 0.0250 | 0.0093 |
| 82.30 | 0.0453 | 0.0491 | 0.0472 | 0.0315 |
| 246.91 | 0.1061 | 0.1035 | 0.1048 | 0.0891 |
| 740.74 | 0.2701 | 0.2723 | 0.2712 | 0.2555 |
| 2222.22 | 0.7935 | 0.7777 | 0.7856 | 0.7699 |
| 6666.67 | 2.0410 | 2.0280 | 2.0345 | 2.0188 |
| 20000.00 | 3.9180 | 3.9010 | 3.9095 | 3.8938 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 370.1 | 1850.1 | 6148.8 | 356.9 | 1784.4 | 6135.0 |
| Standard Deviation | 10.2 | 87.9 | 204.1 | 18.4 | 88.2 | 389.3 |
| Coefficient of Variation (%) | 2.8 | 4.8 | 3.3 | 5.2 | 4.9 | 6.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human uPAR into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 103% with an overall mean recovery of 96%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1694.27-8807.19 | 100 | 3895.95 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of uPAR in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
CD 87
CD87
CD87 antigen
MO 3
MO3
Monocyte activation antigen Mo3
Plasminogen activator receptor urokinase
Plasminogen activator urokinase receptor
PLAUR
U PAR
展開