Human TFF1/Trefoil Factor 1 enzyme-linked immunoassay kit
Catalog# EH0333
Human TFF1/Trefoil Factor 1 enzyme-linked immunoassay kit
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sELISA
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Human
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46.88 ~3000 pg/ml
製品概要
製品名
Human TFF1/Trefoil Factor 1 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
2.63pg/ml (50 μl)；6.94 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0085 | 0.0078 | 0.0082 | |
| 46.88 | 0.0610 | 0.0599 | 0.0605 | 0.0523 |
| 93.75 | 0.1348 | 0.1394 | 0.1371 | 0.1290 |
| 187.50 | 0.3557 | 0.3418 | 0.3488 | 0.3406 |
| 375.00 | 0.8953 | 0.8953 | 0.8953 | 0.8872 |
| 750.00 | 2.0220 | 2.0150 | 2.0185 | 2.0104 |
| 1500.00 | 3.5550 | 3.5070 | 3.5310 | 3.5229 |
| 3000.00 | 4.3890 | 4.2780 | 4.3335 | 4.3254 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 44.6 | 257.0 | 805.5 | 44.9 | 258.4 | 802.3 |
| Standard Deviation | 2.0 | 13.4 | 35.3 | 2.0 | 10.2 | 34.9 |
| Coefficient of Variation (%) | 4.4 | 5.2 | 4.4 | 4.4 | 3.9 | 4.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human TFF1/Trefoil Factor 1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 70% to 112% with an overall mean recovery of 96%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 365.62-3143.92 | 100 | 852.50 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human TFF1/Trefoil Factor 1 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
Trefoil factor 1
Breast cancer estrogen-inducible protein
PNR-2
Polypeptide P1.A (hP1.A)
Protein pS2
TFF1
BCEI, PS2