Human beta Amyloid 1-40 enzyme-linked immunoassay kit
Catalog# EH0111
Human beta Amyloid 1-40 enzyme-linked immunoassay kit
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ELISA
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Human
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31.25~2000 pg/ml
製品概要
製品名
Human beta Amyloid 1-40 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
2.92 pg/ml (50 μl)；8.40 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0446 | 0.0451 | 0.0449 | |
| 31.25 | 0.0817 | 0.0770 | 0.0794 | 0.0345 |
| 62.50 | 0.1291 | 0.1349 | 0.1320 | 0.0871 |
| 125.00 | 0.2772 | 0.2812 | 0.2792 | 0.2343 |
| 250.00 | 0.5233 | 0.5129 | 0.5181 | 0.4732 |
| 500.00 | 1.0246 | 1.0388 | 1.0317 | 0.9868 |
| 1000.00 | 1.8213 | 1.8056 | 1.8135 | 1.7686 |
| 2000.00 | 3.3130 | 3.3240 | 3.3185 | 3.2736 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 757.9 | 168.5 | 48.0 | 703.8 | 202.7 | 42.7 |
| Standard Deviation | 40.9 | 7.1 | 1.8 | 22.1 | 5.6 | 2.4 |
| Coefficient of Variation (%) | 5.4 | 4.2 | 3.7 | 3.1 | 2.8 | 5.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human beta Amyloid 1-40 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 122% with an overall mean recovery of 105%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) | |
|---|---|---|---|---|---|
| Serum | 30 | n.d.- | 907.22 | 37.5 | 198.85 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of beta Amyloid 1-40 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存