Human MBL2 enzyme-linked immunoassay kit

見積もりを依頼する

お問い合わせ

E-mail: [email protected]

TEL: 0571-88062880

代理店・販売店を探す

Catalog# EH0090

Human MBL2 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

0.03~20 ng/ml

製品概要

製品名

Human MBL2 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

1.92 pg/ml (50 μl)

ng/ml	O.D.		Average	Corrected
0.00	0.0068	0.0089	0.0079
0.03	0.0184	0.0191	0.0188	0.0109
0.08	0.0431	0.0464	0.0448	0.0369
0.25	0.1218	0.1243	0.1231	0.1152
0.74	0.3541	0.3709	0.3625	0.3547
2.22	0.9895	1.0440	1.0168	1.0089
6.67	2.2540	2.3080	2.2810	2.2732
20.00	3.8100	3.8060	3.8080	3.8002

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	5558.1	1836.6	373.7	5910.8	1881.4	382.7
Standard Deviation	194.1	55.8	15.7	171.4	52.1	9.5
Coefficient of Variation (%)	3.5	3.0	4.2	2.9	2.8	2.5

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human MBL2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 89% to 118% with an overall mean recovery of 102%.

サンプル値

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	154.5-547.4	100	293.8

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of MBL2 in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

75min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P11226 Human

別名

COLEC 1

COLEC1

Collectin-1

HSMBPC

Lectin

mannose-binding

soluble

Mannan binding lectin

Mannan binding protein

展開

機能

Mannan binding lectin (MBL) belongs to the collectin family of innate immune defense proteins, which binds to an array of carbohydrate patterns on pathogen surfaces. Collectin family members share common structural features: a cysteine rich amino-terminal domain, a collagen-like region, an alpha -helical coiled-coil neck domain and a carboxy terminal C-type (Ca++-dependent) lectin or carbohydrate recognition domain (CRD). MBL homotrimerizes to form a structural unit joined by N-terminal disulfide bridges. These homotrimers further associates into oligomeric structures of up to 6 units. Whereas two forms of MBL proteins (MBL-1, also known as S-MBP or MBL-A and MBL-2, also known as L-MBP or MBL-C) exist in rodents and other animals, only one functional MBL protein is present in humans. Mouse MBL-2 shares about 52% and 60% amino acid sequence identity with mouse MBL-1 and human MBL, respectively. In mouse, MBL-1 and MBL-2 are the only collectins that can activate complement via the lectin complement pathway. Serum oligomeric MBL associates with MBL-associated serine protease (MASP) proenzymes. The MBL-MASP proenzyme complex preferentially interact with sugar patterns containing mannose, glucose, L‑fucose, or N-acetyl-glucosamine present at a terminal nonreducing postion on the cell surface of various pathogens and certain tumor cells. This interaction induces pro-enzyme activation and the triggering of the complement cascade, resulting in opsonization and pathogen removal via humoral and cellular immune responses. MBL does not recognize self-components or glycoproteins from other higher animals due to the presence of terminal sialic acid or galactose that interrupts the repeating carbohydrate structures. A number of membrane receptors for MBL, including C1q phagocytic receptor (C1qRp), calreticulin (also known as C1qR), and CR1 (CD35), have been described. Interactions with these receptors may also be important in stimulating phagocytosis. Mouse MBL-1 and MBL-2 are produced primarily in the liver and are secreted into the blood stream. In addition, mouse MBL-1 is also expressed in lung, kidney, and testis while MBL-2 is expressed in kidney, thymus, and small intestine.

Human MBL2 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

同一ターゲット・同一パスウェイ製品

Human CD47 enzyme-linked immunoassay kit

Human beta Amyloid 1-42 enzyme-linked immunoassay kit

Human/Mouse MASP2 enzyme-linked immunoassay kit

Human SAA1 enzyme-linked immunoassay kit

Human CD254/RANKL/TNFSF11 enzyme-linked immunoassay kit

Human IL-3 enzyme-linked immunoassay kit

Human Complement factor B enzyme-linked immunoassay kit(one step)

Human IL-17E/IL-25 enzyme-linked immunoassay kit

Human Myeloperoxidase(MPO) enzyme-linked immunoassay kit(one step)

Human Leptin enzyme-linked immunoassay kit

Human PAI-1/Serpin E1 enzyme-linked immunoassay kit(one step)

Human ULBP1/NKG2DL1 enzyme-linked immunoassay kit

Human CD273/B7-DC/PD-L2 enzyme-linked immunoassay kit（one step）

Human VE Cadherin enzyme-linked immunoassay kit

Human CD62E enzyme-linked immunoassay kit

Human/Monkey alpha Fetoprotein enzyme-linked immunoassay kit

Human CX3CL1/Fractalking enzyme-linked immunoassay kit

Human/Monkey PCSK9 enzyme-linked immunoassay kit (one step)

Human Growth Hormone enzyme-linked immunoassay kit

Human Nectin-2 enzyme-linked immunoassay kit

Human/Monkey/Canine Osteopontin（OPN） enzyme-linked immunoassay kit

Human Total HSP60 enzyme-linked immunoassay kit

Human Angiotensinogen enzyme-linked immunoassay kit

Human IL-33 enzyme-linked immunoassay kit

Human RAGE enzyme-linked immunoassay kit（one step）

Human Prostate specific Antigen/KLK3 enzyme-linked immunoassay kit

Human CD48 enzyme-linked immunoassay kit

Human/Monkey Albumin enzyme-linked immunoassay kit(one step)

Human IL-13 enzyme-linked immunoassay kit

Human B7-H2/CD275 enzyme-linked immunoassay kit

Human Alpha-Synuclein enzyme-linked immunoassay kit

Human MMP-3 enzyme-linked immunoassay kit

Human MERTK enzyme-linked immunoassay kit（one step）

Human Adiponectinenzyme-linked immunoassay kit（one step）

Human CCL25 enzyme-linked immunoassay kit

Human TREM-1 enzyme-linked immunoassay kit

Human Lipoprotein A/LPA enzyme-linked immunoassay kit(one step)

Human TNF RI/TNFRSF1A enzyme-linked immunoassay kit

Human Lipoprotein A/LPA enzyme-linked immunoassay kit

Human MICB enzyme-linked immunoassay kit

Human SCF/KITLG enzyme-linked immunoassay kit

Human CCL17/TARC enzyme-linked immunoassay kit

Human/Monkey/Canine IL-18 enzyme-linked immunoassay kit

Human tPA enzyme-linked immunoassay kit(one step)

Human Antithrombin III/AT III enzyme-linked immunoassay kit(one step)

Human TSLP enzyme-linked immunoassay kit

Human/Monkey Clusterin enzyme-linked immunoassay kit(one step)

Human FGF-19 enzyme-linked immunoassay kit

Human/Monkey Complement C3 enzyme-linked immunoassay kit(one step)

Human CD33 enzyme-linked immunoassay kit

Human Oxytocin-neurophysin 1/OXT enzyme-linked immunoassay kit

Human CXCL5/ENA-78 enzyme-linked immunoassay kit

Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit

Human CTLA-4 enzyme-linked immunoassay kit

Human CHIT1 enzyme-linked immunoassay kit

Human Angiogenin enzyme-linked immunoassay kit（one step）