Human Osteoprotegerin/TNFRSF11B enzyme-linked immunoassay kit
Catalog# EH0070
Human Osteoprotegerin/TNFRSF11B enzyme-linked immunoassay kit
-
ELISA
-
Human
-
6.86~5000 pg/ml
-
unconjugated
製品概要
製品名
Human Osteoprotegerin/TNFRSF11B enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.37 pg/ml (50 μl)；2.53 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0149 | 0.0139 | 0.0144 | |
| 6.86 | 0.0332 | 0.0309 | 0.0321 | 0.0171 |
| 20.58 | 0.0686 | 0.0681 | 0.0684 | 0.0534 |
| 61.73 | 0.1782 | 0.1770 | 0.1776 | 0.1626 |
| 185.19 | 0.4947 | 0.4959 | 0.4953 | 0.4803 |
| 555.56 | 1.2700 | 1.2650 | 1.2675 | 1.2525 |
| 1666.67 | 2.6800 | 2.5420 | 2.6110 | 2.5960 |
| 5000.00 | 3.8430 | 3.6970 | 3.7700 | 3.7550 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 194.3 | 749.0 | 1558.3 | 209.4 | 735.1 | 1459.6 |
| Standard Deviation | 12.2 | 35.0 | 105.3 | 9.6 | 24.8 | 29.6 |
| Coefficient of Variation (%) | 6.3 | 4.7 | 6.8 | 4.6 | 3.4 | 2.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Osteoprotegerin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 65% to 98% with an overall mean recovery of 95%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) | |
|---|---|---|---|---|---|
| Serum | 30 | 341.28- | 586.42 | 100 | 461.05 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Osteoprotegerin in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
MGC29565
OCIF
OPG
Osteoclastogenesis inhibitory factor
Osteoprotegerin
PDB5
TNF receptor superfamily member 11b
TNFRSF 11B
TNFRSF11B
TR 1
展開