Human IL-5 enzyme-linked immunoassay kit
Catalog# EH0008
Human IL-5 enzyme-linked immunoassay kit
-
ELISA
-
Human
-
1.37~1000 pg/ml
-
unconjugated
製品概要
製品名
Human IL-5 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.15 pg/ml (50 μl) ；0.73 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0 | 0.0324 | 0.0303 | 0.0314 | |
| 1.37 | 0.0392 | 0.0346 | 0.0369 | 0.0056 |
| 4.12 | 0.0484 | 0.0466 | 0.0475 | 0.0162 |
| 12.35 | 0.0832 | 0.0818 | 0.0825 | 0.0512 |
| 37.04 | 0.1765 | 0.1662 | 0.1714 | 0.14 |
| 111.11 | 0.4454 | 0.4245 | 0.435 | 0.4036 |
| 333.33 | 1.1039 | 1.0797 | 1.0918 | 1.0605 |
| 1000 | 2.8645 | 2.8548 | 2.8597 | 2.8283 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 16.3 | 77.9 | 275.8 | 16.4 | 79.9 | 291.5 |
| Standard Deviation | 0.4 | 2.4 | 11.7 | 0.3 | 1.7 | 8.9 |
| Coefficient of Variation (%) | 2.7 | 3.1 | 4.2 | 2.1 | 2.1 | 3.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human IL-5 into health human serum sample. The un-spiked serum was used as blank in these experiments.
The recovery ranged from 88% to 115% with an overall mean recovery of 106%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-6.72 | 21 | 2.13 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-10 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
B-cell differentiation factor I
Colony stimulating factor
EDF
Eosinophil differentiation factor
IL-5
IL5
IL5_HUMAN
Interleukin 5 (colony stimulating factor, eosinophil)
Interleukin 5
Interleukin-5
展開