Human EphA2 enzyme-linked immunoassay kit
Catalog# EH0193
Human EphA2 enzyme-linked immunoassay kit
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ELISA
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Human
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2.74~2000 pg/ml
製品概要
製品名
Human EphA2 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.30 pg/ml (50 μl)；1.23 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0096 | 0.0093 | 0.0095 | |
| 2.74 | 0.0181 | 0.0170 | 0.0176 | 0.0081 |
| 8.23 | 0.0356 | 0.0318 | 0.0337 | 0.0243 |
| 24.69 | 0.0857 | 0.0837 | 0.0847 | 0.0753 |
| 74.07 | 0.2362 | 0.2246 | 0.2304 | 0.2210 |
| 222.22 | 0.6780 | 0.6623 | 0.6702 | 0.6607 |
| 666.67 | 1.6000 | 1.5570 | 1.5785 | 1.5691 |
| 2000.00 | 3.0380 | 3.0070 | 3.0225 | 3.0131 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 48.5 | 221.8 | 684.9 | 40.3 | 204.8 | 657.6 |
| Standard Deviation | 3.2 | 16.2 | 29.7 | 2.9 | 5.7 | 27.7 |
| Coefficient of Variation (%) | 6.5 | 7.3 | 4.3 | 7.2 | 2.8 | 4.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human EphA2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 92% to 102% with an overall mean recovery of 96%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 63.79-146.37 | 100 | 91.12 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of EphA2 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存