Human CKMB enzyme-linked immunoassay kit

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Catalog# EH0072

Human CKMB enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

0.04~30 ng/ml

Conjugation

unconjugated

製品概要

製品名

Human CKMB enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.005 pg/ml (50 μl)；0.07 ng/ml (10 μl)

ng/ml	O.D.		Average	Corrected
0.00	0.0144	0.0158	0.0151
0.04	0.0332	0.0309	0.0321	0.0170
0.12	0.0716	0.0726	0.0721	0.0570
0.37	0.1778	0.1780	0.1779	0.1628
1.11	0.4794	0.4464	0.4629	0.4478
3.33	1.3260	1.3850	1.3555	1.3404
10.00	3.2180	3.2230	3.2205	3.2054
30.00	4.5063	4.4600	4.4832	4.4681

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (ng/ml)	0.7	3.8	13.1	0.6	3.2	11.0
Standard Deviation	0.1	0.2	1.0	0.1	0.1	0.3
Coefficient of Variation (%)	5.6	4.6	7.3	3.6	3.9	3.1

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human CKMB into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 79% to 119% with an overall mean recovery of 103%.

サンプル値

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	n.d.-0.06	40	0.02

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CKMB in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt:P06732

SwissProt:P12277

別名

A2MR

Alpha 2 macroglobulin receptor

alpha 2MR

Alpha-2-macroglobulin receptor

APOER

Apolipoprotein E receptor

APR

CD 91

CD91

CD91 antigen

展開

機能

The creatine kinase molecule is a dimer composed of a brain-type subunit (B) and a muscle-type subunit (M). There are usually three isozymes in normal human tissues, which are CK-BB (CK1), CK-MB (CK2) and CK-MM (CK3) in the order of electrophoresis. CK-BB is mainly found in smooth muscle and brain tissue, and almost none in serum; CK-MB mainly exists in myocardial tissue, accounting for 0~4% of CK in serum; CK-MM is mainly present in skeletal muscle, accounting for 96%~100% of CK in serum. CK-MB has its own subtypes, including MB2 (tissue form) and MB1 (transformed form). After the release of CK-MB2 from the myocardium, the carboxy-terminus is hydrolyzed by plasma carboxypeptidase N, and a lysine residue is removed to be converted to the serum-modified isoform CK-MB1. CK and CK-MB are one of the sensitive indicators of myocardial tissue injury, especially CK-MB is more specific. The increase in CK during myocardial injury is mainly due to the increase in CK-MB, which is manifested by the simultaneous increase of both.

Human CKMB enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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