Human CD66e/CEACAM-5 enzyme-linked immunoassay kit

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Catalog# EH0318

Human CD66e/CEACAM-5 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

15.63 ~1000 pg/ml

製品概要

製品名

Human CD66e/CEACAM-5 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

2.60 pg/ml (50 ul)

pg/ml	O.D.		Average	Corrected
0.00	0.0397	0.0395	0.0396
15.63	0.1158	0.1122	0.1140	0.0744
31.25	0.1874	0.1708	0.1791	0.1395
62.50	0.3159	0.3148	0.3154	0.2758
125.00	0.5713	0.5489	0.5601	0.5205
250.00	1.0140	0.9802	0.9971	0.9575
500.00	1.6900	1.7070	1.6985	1.6589
1000.00	2.8040	2.7650	2.7845	2.7449

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	19.1	99.5	302.4	22.6	98.6	275.8
Standard Deviation	1.2	3.8	12.9	1.0	3.9	11.3
Coefficient of Variation (%)	6.2	3.8	4.3	4.4	3.9	4.1

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human CD66e/CEACAM5 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 99% to 115% with an overall mean recovery of 105%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
serum	30	35.50-300.61	100.0	112.69

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human CD66e/CEACAM5 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P06731 Human

機能

CEACAM-5, also known as CEA and CD66e, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM molecules are either transmembrane or GPI-linked, and are differentially expressed between species. Orthologs of human CEACAM‑5 have not been described in other species. CEACAM-5, which is expressed primarily by epithelial cells, consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor. CEACAM-5 is synthesized as a 180 kDa, variably glycosylated molecule of which approximately 60% is carbohydrate. CEACAM-5 functions as a calcium‑independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1. CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors. This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is upregulated in a wide variety of human tumors and is a commonly used cancer marker. It promotes tumor cell migration, invasion, adhesion, and metastasis. It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis). The GPI anchoring of CEACAM-5 can be released by GPI-PLD, resulting in a soluble molecule that also promotes tumor metastasis. Cell surface expression of CEACAM-5 on tumor cells prevents the adhesion of CEACAM-1 expressing NK cells and provides protection from NK‑mediated lysis. CEACAM-5 also binds a subset of Neisseria opacity proteins (Opa) and E. coli adhesion proteins. These interactions trigger clustering of the lipid raft-localized CEACAM-5 to sites of pathogen contact.

Human CD66e/CEACAM-5 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

機能

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