Human CD40 enzyme-linked immunoassay kit

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Catalog# EH0280

Human CD40 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

1.37~1000 pg/ml

製品概要

製品名

Human CD40 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.34 pg/ml (50 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0222	0.0225	0.0224
1.37	0.0257	0.0279	0.0268	0.0045
4.12	0.0406	0.0415	0.0411	0.0187
12.35	0.0851	0.0860	0.0856	0.0633
37.04	0.2245	0.2166	0.2206	0.1982
111.11	0.6181	0.6157	0.6169	0.5946
333.33	1.7090	1.7460	1.7275	1.7052
1000.00	3.8810	3.9440	3.9125	3.8902

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	23.6	121.8	373.3	23.7	115.7	342.3
Standard Deviation	0.5	2.8	8.2	1.0	3.0	12.5
Coefficient of Variation (%)	2.1	2.3	2.2	4.1	2.6	3.7

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human CD40 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 91% to 121% with an overall mean recovery of 110%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	208.10-361.52	100	281.37

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human CD40 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P25942 Human

機能

CD40 is a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. The mature hCD40 consists of a 172 amino acid (aa) extracellular domain, a 22 aa transmembrane region and a 62 aa cytoplasmic domain. Human and mouse CD40 share 62% aa identity. CD40 is expressed in B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells, and several tumor cell lines. The extracellular domain has the cysteine-rich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to aggregation of CD40 molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Interaction between CD40L on T cells and CD40 on B cells stimulated B cell proliferation and provided the signal for immunoglobulin isotype switching. Mutations in the CD40L gene, which resulted in a CD40L molecule unable to interact with CD40, are responsible for the hyper-IgM syndrome. Cross-linking of CD40 with antibodies or by CD40 binding to CD40L produces cell type-specific responses which include costimulation and induction of proliferation, induction of cytokine production, rescue from apoptosis, and upregulation of adhesion molecules. Some of the early events of intracellular signaling by the CD40‑CD40L system include the association of the CD40 with TRAFs and the activation of various kinases.

Human CD40 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

機能

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