Human Complement C5 enzyme-linked immunoassay kit
Catalog# EH0108
Human Complement C5 enzyme-linked immunoassay kit
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ELISA
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Human
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2.74~2000 pg/ml
製品概要
製品名
Human Complement C5 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.05 pg/ml (50 μl)；3.14 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0191 | 0.0181 | 0.0186 | |
| 2.74 | 0.0494 | 0.0480 | 0.0487 | 0.0301 |
| 8.23 | 0.0899 | 0.0917 | 0.0908 | 0.0722 |
| 24.69 | 0.1898 | 0.1834 | 0.1866 | 0.1680 |
| 74.07 | 0.4362 | 0.4138 | 0.4250 | 0.4064 |
| 222.22 | 0.9751 | 0.9995 | 0.9873 | 0.9687 |
| 666.67 | 2.2140 | 2.1390 | 2.1765 | 2.1579 |
| 2000.00 | 3.8600 | 3.8120 | 3.8360 | 3.8174 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 17.8 | 92.6 | 287.7 | 16.6 | 85.8 | 261.2 |
| Standard Deviation | 0.7 | 4.5 | 15.2 | 0.7 | 4.8 | 16.9 |
| Coefficient of Variation (%) | 3.7 | 4.9 | 5.3 | 4.5 | 5.6 | 6.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
he spike recovery was evaluated by spiking 3 levels of human Complement C5 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 101% with an overall mean recovery of 92%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.79-58.93 | 100 | 25.41 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Complement C5 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
C5
Complement C5
Complement component 5
CPAMD4