Human CNTF enzyme-linked immunoassay kit
Catalog# EH0147
Human CNTF enzyme-linked immunoassay kit
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ELISA
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Human
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2.74~2000 pg/ml
製品概要
製品名
Human CNTF enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.25 pg/ml (50 μl)；3.77 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0328 | 0.0323 | 0.0326 | |
| 2.74 | 0.0495 | 0.0492 | 0.0494 | 0.0168 |
| 8.23 | 0.0814 | 0.0796 | 0.0805 | 0.0480 |
| 24.69 | 0.1741 | 0.1868 | 0.1805 | 0.1479 |
| 74.07 | 0.4648 | 0.4985 | 0.4817 | 0.4491 |
| 222.22 | 1.2410 | 1.3150 | 1.2780 | 1.2455 |
| 666.67 | 3.0640 | 3.2330 | 3.1485 | 3.1160 |
| 2000.00 | 4.5481 | 4.6198 | 4.5840 | 4.5514 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 39.2 | 197.9 | 618.1 | 37.0 | 199.0 | 601.7 |
| Standard Deviation | 1.7 | 8.3 | 30.7 | 2.2 | 14.8 | 39.7 |
| Coefficient of Variation (%) | 4.4 | 4.2 | 5.0 | 6.0 | 7.4 | 6.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human CNTF into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 74% to 112% with an overall mean recovery of 96%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-81.17 | 13 | 42.58 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CNTF in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
Ciliary neurotrophic factor
CNTF
CNTF_HUMAN
HCNTF