Human CCL24/Eotaxin-2/MPIF-2 enzyme-linked immunoassay kit

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Catalog# EH0283

Human CCL24/Eotaxin-2/MPIF-2 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

31.25~2000 pg/ml

製品概要

製品名

Human CCL24/Eotaxin-2/MPIF-2 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.75 pg/ml (50 μl)；9.71 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0422	0.0372	0.0397
31.25	0.0914	0.0879	0.0897	0.0500
62.50	0.1376	0.1466	0.1421	0.1024
125.00	0.2545	0.2710	0.2628	0.2231
250.00	0.5244	0.5137	0.5191	0.4794
500.00	1.0090	1.0250	1.0170	0.9773
1000.00	2.0390	2.0250	2.0320	1.9923
2000.00	3.3060	3.3210	3.3135	3.2738

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	341.7	1933.2	5656.0	396.7	1924.8	5724.1
Standard Deviation	25.4	50.6	153.1	30.8	36.8	93.0
Coefficient of Variation (%)	7.4	2.6	2.7	7.8	1.9	1.6

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human CCL24/Eotaxin-2/MPIF-2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 105% to 126% with an overall mean recovery of 108%.

サンプル値

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	137.98-767.80	100.0	377.41

Serum/Plasma– Thirty samples from apparently healthy volunteers were evaluated for the presence of human CCL24/Eotaxin-2/MPIF-2 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: O00175 Human

機能

Eotaxin, also named MPIF-2 and Ck beta 6, is a novel CC chemokine identified in the Human Genome Sciences, Inc. database based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin-2 cDNA encodes a 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. Although one recombinant preparation of MPIF‑2/Eotaxin‑2 from insect cells was reported to be glycosylated and of the predicted size (10.5 kDa), a second preparation of recombinant Eotaxin-2 prepared by a different group, also from insect cells, was shown to contain a 78 amino acid residue carboxy-terminally truncated variant of Eotaxin-2. Additional minor carboxy‑terminally truncated variants with 73, 75 and 76 residues were also isolated. Compared to other CC chemokines, Eotaxin-2 exhibits 40%, 42% and 39% amino acid identity to MCP-3, MIP-1 alpha, and Eotaxin, respectively. Eotaxin-2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant Eotaxin-2 has been shown to induce chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin-2 has also been shown to suppress the colony formation by the high proliferative potential colony-forming cells which represent multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin-2 was shown to be inhibited by an anti-CCR-3 antibody and to be cross-desensitized by Eotaxin or MCP-4, suggesting that all three CC chemokines act through CCR-3, at least on eosinophils.

Human CCL24/Eotaxin-2/MPIF-2 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

機能

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