Human S100A12 enzyme-linked immunoassay kit(one step)
Catalog# EHY0229
Human S100A12 enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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0.14~100 ng/ml
製品概要
製品名
Human S100A12 enzyme-linked immunoassay kit(one step)
製品タイプ
Elisa Kit
検出感度
0.004 ng/ml (50 μl)；0.05 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0079 | 0.0076 | 0.0078 | |
| 0.14 | 0.0318 | 0.0338 | 0.0328 | 0.0251 |
| 0.41 | 0.1116 | 0.1126 | 0.1121 | 0.1044 |
| 1.23 | 0.3195 | 0.3044 | 0.3120 | 0.3042 |
| 3.70 | 0.8767 | 0.8829 | 0.8798 | 0.8721 |
| 11.11 | 2.2750 | 2.2900 | 2.2825 | 2.2748 |
| 33.33 | 3.5036 | 3.5559 | 3.5298 | 3.5220 |
| 100.00 | 4.4908 | 4.7563 | 4.6236 | 4.6158 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 2.1 | 11.2 | 32.4 | 2.3 | 10.9 | 30.2 |
| Standard Deviation | 0.1 | 0.4 | 2.2 | 0.1 | 0.4 | 1.0 |
| Coefficient of Variation (%) | 4.8 | 3.6 | 6.8 | 4.3 | 3.7 | 3.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of Human S100A12 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 118% with an overall mean recovery of 105%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 16.60-62.08 | 100 | 39.18 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存