Human Renin enzyme-linked immunoassay kit
Catalog# EH0211
Human Renin enzyme-linked immunoassay kit
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ELISA
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Human
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2.74~2000 pg/ml
製品概要
製品名
Human Renin enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.08 pg/ml (50 μl)；0.25 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0078 | 0.0077 | 0.0077 | |
| 2.74 | 0.0338 | 0.0321 | 0.0330 | 0.0253 |
| 8.23 | 0.0883 | 0.0978 | 0.0931 | 0.0854 |
| 24.69 | 0.2698 | 0.2577 | 0.2638 | 0.2561 |
| 74.07 | 0.7291 | 0.6849 | 0.7070 | 0.6993 |
| 222.22 | 1.7700 | 1.6890 | 1.7295 | 1.7218 |
| 666.67 | 3.1820 | 3.0870 | 3.1345 | 3.1268 |
| 2000.00 | 4.1599 | 4.1959 | 4.1779 | 4.1702 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 36.2 | 188.8 | 754.8 | 37.5 | 191.8 | 651.7 |
| Standard Deviation | 1.0 | 8.4 | 29.6 | 1.7 | 5.8 | 17.0 |
| Coefficient of Variation (%) | 2.8 | 4.4 | 3.9 | 4.6 | 3.0 | 2.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Renin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 120% with an overall mean recovery of 106%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 415.85-1298.17 | 100 | 791.38 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Renin in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
Angiotensin forming enzyme
Angiotensin forming enzyme precursor
Angiotensinogenase
Angiotensinogenase precursor
FLJ10761
HNFJ2
REN
Ren1
RENI_HUMAN
Renin
展開