Human IL-17F enzyme-linked immunoassay kit
Catalog# EH0249
Human IL-17F enzyme-linked immunoassay kit
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ELISA
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Human
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1.37~1000 pg/ml
製品概要
製品名
Human IL-17F enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.60 pg/ml (50 μl)；1.17 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0201 | 0.0188 | 0.0188 | |
| 1.37 | 0.0255 | 0.0254 | 0.0255 | 0.0067 |
| 4.12 | 0.0396 | 0.0393 | 0.0395 | 0.0207 |
| 12.35 | 0.0677 | 0.0694 | 0.0686 | 0.0498 |
| 37.04 | 0.1612 | 0.1615 | 0.1614 | 0.1426 |
| 111.11 | 0.4124 | 0.4180 | 0.4152 | 0.3964 |
| 333.33 | 1.0830 | 1.2010 | 1.1420 | 1.1232 |
| 1000.00 | 2.6840 | 2.8360 | 2.7600 | 2.7412 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 15.0 | 88.2 | 294.2 | 18.8 | 98.7 | 302.5 |
| Standard Deviation | 1.2 | 6.9 | 18.9 | 0.5 | 4.7 | 3.5 |
| Coefficient of Variation (%) | 7.8 | 7.8 | 6.4 | 2.7 | 4.8 | 1.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human IL-17F into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 79% to 102% with an overall mean recovery of 93%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-13.94 | 25 | 4.76 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存