Human TRAIL/TNFSF10 enzyme-linked immunoassay kit

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Catalog# EH0176

Human TRAIL/TNFSF10 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

8.19~2000 pg/ml

製品概要

製品名

Human TRAIL/TNFSF10 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.90 pg/ml (50 μl)；11.03 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0079	0.0090	0.0085
8.19	0.0236	0.0233	0.0235	0.0150
20.48	0.0491	0.0480	0.0486	0.0401
51.20	0.1139	0.1073	0.1106	0.1022
128.00	0.2820	0.2697	0.2759	0.2674
320.00	0.6811	0.6529	0.6670	0.6586
800.00	1.4760	1.4190	1.4475	1.4391
2000.00	2.7240	2.6890	2.7065	2.6981

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	76.8	298.2	834.6	72.7	285.1	730.8
Standard Deviation	3.5	9.6	26.2	3.1	8.3	24.7
Coefficient of Variation (%)	4.5	3.2	3.1	4.3	2.9	3.4

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human TRAIL/TNFSF10 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 120% with an overall mean recovery of 109%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	3.12-119.35	100	64.97

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of TRAIL/TNFSF10 in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P50591 Human

別名

Apo 2 ligand

APO 2L

Apo-2 ligand

Apo-2L

APO2L

CD253

CD253 antigen

Chemokine tumor necrosis factor ligand superfamily member 10

Protein TRAIL

TL2

展開

機能

TRAIL (TNF-related apoptosis-inducing ligand), also known as APO-2 ligand, is a type II transmembrane protein with a carboxy-terminal extracellular domain which exhibits homology to other TNF family members. In the new TNF superfamily nomenclature, TRAIL is referred to as TNFSF10. Human TRAIL cDNA encodes a 281 amino acid (aa) residue protein with an amino-terminal intracellular domain of 17 residues and a predicted internal hydrophobic domain between residues 18 and 38. The extracellular carboxy-terminal domain contains a potential N-linked glycosylation site at amino acid residue 109. Among TNF family members, TRAIL is most homologous to FAS ligand, sharing 28% amino acid sequence identity in their extracellular domains. Mouse TRAIL has also been cloned. The human TRAIL shares 65% amino acid sequence identity with mouse TRAIL and is active on mouse cells. Both membrane bound and soluble TRAIL have been shown to induce rapid apoptosis of many transformed cell lines. Like most TNF family members, the bioactive TRAIL exists as a homotrimer. TRAIL transcripts have been shown to be constitutively expressed in a variety of human tissues. A family of TRAIL receptors, including two receptors that tranduce the apoptotic signals and two TRAIL decoy receptors that function to antagonize TRAIL-induced apoptosis, have been identified. Osteoprotegerin has been identified as a fifth TRAIL receptor.

Human TRAIL/TNFSF10 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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