Human Angiogenin enzyme-linked immunoassay kit（one step）
Catalog# EHY0190
Human Angiogenin enzyme-linked immunoassay kit（one step）
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ELISA
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Human
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0.41~100 ng/ml
製品概要
製品名
Human Angiogenin enzyme-linked immunoassay kit（one step）
製品タイプ
Elisa Kit
検出感度
0.01 ng/ml (50 μl)；0.07 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0123 | 0.0123 | 0.0123 | |
| 0.41 | 0.0423 | 0.0436 | 0.0430 | 0.0307 |
| 1.02 | 0.0915 | 0.0921 | 0.0918 | 0.0795 |
| 2.56 | 0.2093 | 0.2128 | 0.2111 | 0.1988 |
| 6.40 | 0.5093 | 0.5195 | 0.5144 | 0.5021 |
| 16.00 | 1.2320 | 1.2210 | 1.2265 | 1.2142 |
| 40.00 | 2.5030 | 2.4640 | 2.4835 | 2.4712 |
| 100.00 | 3.4960 | 3.4790 | 3.4875 | 3.4752 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 2.2 | 9.9 | 34.2 | 2.4 | 10.6 | 35.4 |
| Standard Deviation | 0.1 | 0.1 | 0.6 | 0.0 | 0.2 | 0.6 |
| Coefficient of Variation (%) | 2.3 | 1.4 | 1.6 | 1.1 | 1.7 | 1.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Angiogenin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 119% with an overall mean recovery of 96%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 115.46-277.38 | 100 | 168.44 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Angiogenin in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
ALS9
AMYOTROPHIC LATERAL SCLEROSIS
ANG 1
ANG
ANG I
ANG1
ANGI
ANGI_HUMAN
Angiogenin
Angiogenin ribonuclease RNase A family, 5
展開