Human Prolactin enzyme-linked immunoassay kit
Catalog# EH0089
Human Prolactin enzyme-linked immunoassay kit
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ELISA
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Human
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31.25~2000 pg/ml
製品概要
製品名
Human Prolactin enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
3.47 pg/ml (50 μl)；11.20 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0672 | 0.0630 | 0.0651 | |
| 31.25 | 0.1314 | 0.1212 | 0.1263 | 0.0612 |
| 62.50 | 0.1703 | 0.1765 | 0.1734 | 0.1083 |
| 125.00 | 0.3135 | 0.3042 | 0.3089 | 0.2438 |
| 250.00 | 0.5341 | 0.5419 | 0.5380 | 0.4729 |
| 500.00 | 1.0220 | 1.0100 | 1.0160 | 0.9509 |
| 1000.00 | 1.9160 | 1.9370 | 1.9265 | 1.8614 |
| 2000.00 | 3.3550 | 3.4380 | 3.3965 | 3.3314 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 38.9 | 216.2 | 558.7 | 43.9 | 229.2 | 598.9 |
| Standard Deviation | 3.2 | 6.4 | 16.7 | 3.3 | 8.4 | 18.1 |
| Coefficient of Variation (%) | 8.0 | 2.9 | 3.0 | 7.4 | 3.7 | 3.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Prolactin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 115% with an overall mean recovery of 102%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1055.28-7606.60 | 100 | 3687.09 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Prolactin in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
Decidual prolactin
GHA1
Growth hormone A1
Lactogenic hormone
Luteotropic hormone
Mammotropin
PRL
Prolactin
Prolactin precursor