Human Endoglin/CD105 enzyme-linked immunoassay kit

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Catalog# EH0204

Human Endoglin/CD105 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

13.72~10000 pg/ml

製品概要

製品名

Human Endoglin/CD105 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.62 pg/ml (50 μl)；3.96 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0072	0.0064	0.0068
13.72	0.0164	0.0144	0.0154	0.0086
41.15	0.0337	0.0375	0.0356	0.0288
123.46	0.1001	0.0956	0.0979	0.0911
370.37	0.2706	0.2572	0.2639	0.2571
1111.11	0.7276	0.7420	0.7348	0.7280
3333.33	1.6110	1.5820	1.5965	1.5897
10000.00	2.7100	2.7090	2.7095	2.7027

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	232.5	1102.0	3424.1	222.7	1086.2	3344.7
Standard Deviation	8.6	46.3	216.7	10.1	77.0	262.4
Coefficient of Variation (%)	3.7	4.2	6.3	4.5	7.1	7.8

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human Endoglin/CD105 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 110% with an overall mean recovery of 98%.

サンプル値

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	9.88-20.14	100	13.61

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Endoglin/CD105 in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P17813 Human

別名

AI528660

AI662476

CD 105

CD105

CD105 antigen

EGLN_HUMAN

END

Endoglin

Eng

FLJ41744

展開

機能

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins. Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% amino acid (aa) identity within the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells. Mouse Endoglin cDNA encodes 653 aa including a 26 aa signal sequence, a 555 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain. A mouse isoform with a 35 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin. The mouse Endoglin ECD shares 69%, 84%, 62%, 63%, and 66% aa identity with human, rat, bovine, porcine, and canine Endoglin, respectively. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII. Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6. BMP-9, however, is reported to bind Endoglin directly. Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line. In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation. Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I. These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization. In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFlt-1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity.

Human Endoglin/CD105 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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