Human IL-10RB enzyme-linked immunoassay kit
Catalog# EH0223
Human IL-10RB enzyme-linked immunoassay kit
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ELISA
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Human
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2.74~2000 pg/ml
製品概要
製品名
Human IL-10RB enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.17 pg/ml (50 μl)；1.65 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0114 | 0.0103 | 0.0109 | |
| 2.74 | 0.0304 | 0.0268 | 0.0286 | 0.0178 |
| 8.23 | 0.0668 | 0.0605 | 0.0637 | 0.0528 |
| 24.69 | 0.1729 | 0.1688 | 0.1709 | 0.1600 |
| 74.07 | 0.4923 | 0.4784 | 0.4854 | 0.4745 |
| 222.22 | 1.3010 | 1.3120 | 1.3065 | 1.2957 |
| 666.67 | 3.0620 | 3.0470 | 3.0545 | 3.0437 |
| 2000.00 | 4.3703 | 4.3789 | 4.3746 | 4.3638 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 46.3 | 222.3 | 675.7 | 43.3 | 211.1 | 629.1 |
| Standard Deviation | 2.2 | 11 | 37.8 | 1.7 | 11.5 | 30.0 |
| Coefficient of Variation (%) | 4.7 | 5 | 5.6 | 3.8 | 5.4 | 4.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human IL-10RB into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 95% to 126% with an overall mean recovery of 112%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 446.84-786.15 | 100 | 546.67 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存