Human CCL25 enzyme-linked immunoassay kit

見積もりを依頼する

お問い合わせ

E-mail: [email protected]

TEL: 0571-88062880

代理店・販売店を探す

Catalog# EH0117

Human CCL25 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

1.37~1000 pg/ml

製品概要

製品名

Human CCL25 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.06 pg/ml (50 μl)；0.32 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0067	0.0063	0.0065
1.37	0.0212	0.0206	0.0209	0.0144
4.12	0.0535	0.0512	0.0524	0.0459
12.35	0.1545	0.1510	0.1528	0.1463
37.04	0.4450	0.4292	0.4371	0.4306
111.11	1.2110	1.2380	1.2245	1.2180
333.33	2.6520	2.6360	2.6440	2.6375
1000.00	4.0170	4.0352	4.0261	4.0196

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	23.6	103.0	292.7	24.1	115.1	349.9
Standard Deviation	1.0	5.4	18.0	1.3	1.8	19.4
Coefficient of Variation (%)	4.4	5.3	6.2	5.3	1.6	5.5

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human CCL25 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 77% to 98% with an overall mean recovery of 84%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	0.12-5.85	100	3.15

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CCL25 in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: O15444 Human

別名

A130072A22Rik

C-C motif chemokine 25

CCL 25

CCL25

CCL25_HUMAN

Chemokine (C C motif) ligand 25

Chemokine (CC motif) ligand 25

Chemokine C C motif ligand 25

Chemokine CC motif ligand 25

Chemokine TECK

展開

機能

CCL25 (thymus-expressed chemokine) is a novel CC chemokine that is distantly related (approximately 20% amino acid sequence identity) to other CC chemokines. Human CCL25 cDNA encodes a 150 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 127 residue mature protein. Mouse CCL25 cDNA has also been cloned and shown to encode a 144 amino acid protein that exhibits 49% amino acid sequence identity to human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant human and mouse CCL25 have been shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. CCL25 has also been shown to be a specific agonist for the orphan receptor called GPR-9-6, now named CCR9.

Human CCL25 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

同一ターゲット・同一パスウェイ製品

Human CD47 enzyme-linked immunoassay kit

Human beta Amyloid 1-42 enzyme-linked immunoassay kit

Human/Mouse MASP2 enzyme-linked immunoassay kit

Human SAA1 enzyme-linked immunoassay kit

Human CD254/RANKL/TNFSF11 enzyme-linked immunoassay kit

Human IL-3 enzyme-linked immunoassay kit

Human Complement factor B enzyme-linked immunoassay kit(one step)

Human IL-17E/IL-25 enzyme-linked immunoassay kit

Human Myeloperoxidase(MPO) enzyme-linked immunoassay kit(one step)

Human Leptin enzyme-linked immunoassay kit

Human PAI-1/Serpin E1 enzyme-linked immunoassay kit(one step)

Human ULBP1/NKG2DL1 enzyme-linked immunoassay kit

Human CD273/B7-DC/PD-L2 enzyme-linked immunoassay kit（one step）

Human VE Cadherin enzyme-linked immunoassay kit

Human CD62E enzyme-linked immunoassay kit

Human/Monkey alpha Fetoprotein enzyme-linked immunoassay kit

Human CX3CL1/Fractalking enzyme-linked immunoassay kit

Human/Monkey PCSK9 enzyme-linked immunoassay kit (one step)

Human Growth Hormone enzyme-linked immunoassay kit

Human Nectin-2 enzyme-linked immunoassay kit

Human/Monkey/Canine Osteopontin（OPN） enzyme-linked immunoassay kit

Human Total HSP60 enzyme-linked immunoassay kit

Human Angiotensinogen enzyme-linked immunoassay kit

Human IL-33 enzyme-linked immunoassay kit

Human RAGE enzyme-linked immunoassay kit（one step）

Human Prostate specific Antigen/KLK3 enzyme-linked immunoassay kit

Human CD48 enzyme-linked immunoassay kit

Human/Monkey Albumin enzyme-linked immunoassay kit(one step)

Human IL-13 enzyme-linked immunoassay kit

Human B7-H2/CD275 enzyme-linked immunoassay kit

Human Alpha-Synuclein enzyme-linked immunoassay kit

Human MMP-3 enzyme-linked immunoassay kit

Human MERTK enzyme-linked immunoassay kit（one step）

Human Adiponectinenzyme-linked immunoassay kit（one step）

Human CCL25 enzyme-linked immunoassay kit

Human Lipoprotein A/LPA enzyme-linked immunoassay kit(one step)

Human TNF RI/TNFRSF1A enzyme-linked immunoassay kit

Human TREM-1 enzyme-linked immunoassay kit

Human Lipoprotein A/LPA enzyme-linked immunoassay kit

Human tPA enzyme-linked immunoassay kit(one step)

Human MICB enzyme-linked immunoassay kit

Human Antithrombin III/AT III enzyme-linked immunoassay kit(one step)

Human TSLP enzyme-linked immunoassay kit

Human/Monkey Clusterin enzyme-linked immunoassay kit(one step)

Human SCF/KITLG enzyme-linked immunoassay kit

Human CD33 enzyme-linked immunoassay kit

Human CXCL5/ENA-78 enzyme-linked immunoassay kit

Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit

Human CTLA-4 enzyme-linked immunoassay kit

Human CHIT1 enzyme-linked immunoassay kit

Human Angiogenin enzyme-linked immunoassay kit（one step）

Human CCL17/TARC enzyme-linked immunoassay kit

Human CTGF/CCN2 enzyme-linked immunoassay kit(one step)

Human/Monkey/Canine IL-18 enzyme-linked immunoassay kit

Human FGF-19 enzyme-linked immunoassay kit

Human/Monkey Complement C3 enzyme-linked immunoassay kit(one step)