Human FGF basic /FGF2/bFGF enzyme-linked immunoassay kit
Catalog# EH0024
Human FGF basic /FGF2/bFGF enzyme-linked immunoassay kit
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ELISA
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Human
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8.19~2000 pg/ml
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unconjugated
製品概要
製品名
Human FGF basic /FGF2/bFGF enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
1.51 pg/ml (50 μl)；9.93 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0482 | 0.0491 | 0.0487 | |
| 8.19 | 0.0711 | 0.0715 | 0.0713 | 0.0227 |
| 20.48 | 0.1028 | 0.1063 | 0.1046 | 0.0559 |
| 51.20 | 0.1919 | 0.1881 | 0.1900 | 0.1414 |
| 128.00 | 0.4119 | 0.4048 | 0.4084 | 0.3597 |
| 320.00 | 0.9108 | 0.8764 | 0.8936 | 0.8450 |
| 800.00 | 1.9350 | 1.9990 | 1.9670 | 1.9184 |
| 2000.00 | 3.5980 | 3.6550 | 3.6265 | 3.5779 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 36.8 | 179.7 | 464.2 | 31.66 | 170.64 | 581.98 |
| Standard Deviation | 1.4 | 6.9 | 13.8 | 2.38 | 11.59 | 25.24 |
| Coefficient of Variation (%) | 3.9 | 3.8 | 3 | 7.5 | 6.8 | 4.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human FGF into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 78% to 92% with an overall mean recovery of 86%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 5.45-24.74 | 100 | 15.01 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of HGF in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
文献におけるアプリケーション
| ELISA | 下記の<%= count %>篇の文献を見る |
文献におけるアプリケーション
| Mouse | 下記の<%= count %>篇の文献を見る |
| Human | 下記の<%= count %>篇の文献を見る |
ターゲット
UNIPROT #
別名
Basic fibroblast growth factor
Basic fibroblast growth factor bFGF
BFGF
FGF 2
FGF B
FGF-2
Fgf2
FGF2 basic
FGF2_HUMAN
FGFB
展開機能
文献・引用論文
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Inhalable Chemotactic Liposome for Targeted Modulation of Pulmonary Pre-Metastatic Niche
ジャーナル: Advanced Functional Materials
DOI:
IF: 18.5
アプリケーション: ELISA
交差反応性: Mouse
掲載日: 2025 Jan
-
Inhalable Chemotactic Liposome for Targeted Modulation of Pulmonary Pre-Metastatic Niche
ジャーナル: Advanced Functional Materials
DOI: 10.1002/adfm.202419583
IF: 19
アプリケーション: ELISA
交差反応性: Mouse
掲載日: 2025 Jan
-
A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment
ジャーナル: Bioactive Materials
DOI: 10.1016/j.bioactmat.2024.11.005
IF: 18
アプリケーション: ELISA
交差反応性: Human
掲載日: 2024 Dec