Human TRAF1 enzyme-linked immunoassay kit
Catalog# EH0215
Human TRAF1 enzyme-linked immunoassay kit
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ELISA
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Human
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20.48~5000 pg/ml
製品概要
製品名
Human TRAF1 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
2.85 pg/ml (50 μl)；7.62 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0352 | 0.0374 | 0.0363 | |
| 20.48 | 0.0482 | 0.0512 | 0.0497 | 0.0134 |
| 51.20 | 0.0685 | 0.0666 | 0.0676 | 0.0313 |
| 128.00 | 0.1148 | 0.1100 | 0.1124 | 0.0761 |
| 320.00 | 0.2349 | 0.2201 | 0.2275 | 0.1912 |
| 800.00 | 0.5329 | 0.5233 | 0.5281 | 0.4918 |
| 2000.00 | 1.2500 | 1.2230 | 1.2365 | 1.2002 |
| 5000.00 | 2.8350 | 2.7000 | 2.7675 | 2.7312 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 89.7 | 437.9 | 1342.2 | 79.1 | 414.5 | 1371.4 |
| Standard Deviation | 4.9 | 16.7 | 58.5 | 5.9 | 8.8 | 47.9 |
| Coefficient of Variation (%) | 5.5 | 3.8 | 4.4 | 7.4 | 2.1 | 3.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human TRAF1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from72% to 94% with an overall mean recovery of 84%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-43.04 | 63 | 14.45 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of TRAF1 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
4732496E14Rik
EBI6
EBV induced protein 6
Epstein-Barr virus-induced protein 6
MGC:10353
Tnf receptor associated factor 1
TNF receptor-associated factor 1
TRAF 1
TRAF1
TRAF1_HUMAN