Human Prealbumin/Transthyretin enzyme-linked immunoassay kit(one step)
Catalog# EHY0250
Human Prealbumin/Transthyretin enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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3.13~200 ng/ml
製品概要
製品名
Human Prealbumin/Transthyretin enzyme-linked immunoassay kit(one step)
製品タイプ
Elisa Kit
検出感度
0.02 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0095 | 0.0098 | 0.0097 | |
| 3.13 | 0.0895 | 0.0823 | 0.0859 | 0.0763 |
| 6.25 | 0.1597 | 0.1545 | 0.1571 | 0.1475 |
| 12.50 | 0.3028 | 0.3084 | 0.3056 | 0.2960 |
| 25.00 | 0.6030 | 0.5964 | 0.5997 | 0.5901 |
| 50.00 | 1.1280 | 1.1260 | 1.1270 | 1.1174 |
| 100.00 | 1.9260 | 1.8610 | 1.8935 | 1.8839 |
| 200.00 | 3.0040 | 2.9850 | 2.9945 | 2.9849 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 4.2 | 19.3 | 58.0 | 4.5 | 19.9 | 59.2 |
| Standard Deviation | 0.2 | 0.9 | 2.8 | 0.1 | 0.6 | 2.4 |
| Coefficient of Variation (%) | 4.7 | 4.5 | 4.8 | 2.4 | 3.1 | 4.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Prealbumin/Transthyretin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 115% with an overall mean recovery of 97%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 227.44-506.95 | 100 | 378.79 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存