Human CD48 enzyme-linked immunoassay kit

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Catalog# EH0119

Human CD48 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

1.37~1000 pg/ml

製品概要

製品名

Human CD48 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.25 pg/ml (50 μl)；1.05 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0137	0.0135	0.0136
1.37	0.0207	0.0203	0.0205	0.0069
4.12	0.0328	0.0325	0.0327	0.0191
12.35	0.0735	0.0744	0.0740	0.0604
37.04	0.1995	0.1974	0.1985	0.1849
111.11	0.5747	0.5406	0.5577	0.5441
333.33	1.4730	1.6080	1.5405	1.5269
1000.00	3.3010	3.2540	3.2775	3.2639

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	22.9	105.7	348.3	22.5	109.1	338.0
Standard Deviation	0.7	3.3	12.2	1.0	3.3	12.2
Coefficient of Variation (%)	3.2	3.1	3.5	4.4	3.0	3.6

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human CD48 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 109% with an overall mean recovery of 93%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	2395.44-4374.87	100	3246.68

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD48 in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P09326 Human

別名

CD48 antigen

CD48 molecule

CD48

hCD48

SLAMF2

BCM1 surface antigen

BCM1

BCM1Leukocyte antigen MEM-102

BLAST

BLAST1

展開

機能

CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily proteins. The mouse CD48 cDNA encodes a 240 amino acid (aa) precursor that includes a 22 aa signal sequence, a 195 aa mature protein that contains one Ig-like V-type domain and one Ig-like C2-type domain, and a 23 aa C-terminal propeptide. A soluble form of CD48 has been detected in the serum of lymphoid leukemia and arthritis patients. Mouse CD48 shares 51% and 68% aa sequence identity with human and rat CD48, respectively. It shares 18%‑26% aa sequence identity with comparable regions of mouse 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM. CD48 is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. Among dendritic cells (DC), CD48 is selectively expressed on circulating myeloid DC and resident bone marrow and thymus DC. CD2, 2B4, and heparan sulfate function as CD48 ligands. CD48 is competent to transduce signals and can also trigger signaling through CD2 or 2B4. CD48-CD2 interactions promote T cell activation and class switching to IgG2a in B cells. High affinity CD48-2B4 interactions can either promote or inhibit NK cell and cytotoxic T cell (CTL) activation. CD48-2B4 ligation does not directly trigger CTL activity but enhances signaling from the T cell receptor. CD48-2B4 mediated inhibition of NK cell activity is distinct from MHC I-restricted mechanisms. CD48 expressed on NK cells is coactivating, whereas CD48 expressed on other cell types inhibits NK cell activation (14). Both CD48 expressing and nonexpressing cells can be targets of NK cell or CTL-mediated lysis.

Human CD48 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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