Human cIAP2 enzyme-linked immunoassay kit
Catalog# EH0123
Human cIAP2 enzyme-linked immunoassay kit
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ELISA
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Human
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2.74~2000 pg/ml
製品概要
製品名
Human cIAP2 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.81 pg/ml (50 μl)；1.59 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0139 | 0.0145 | 0.0142 | |
| 2.74 | 0.0221 | 0.0234 | 0.0228 | 0.0086 |
| 8.23 | 0.0328 | 0.0302 | 0.0315 | 0.0173 |
| 24.69 | 0.0653 | 0.0689 | 0.0671 | 0.0529 |
| 74.07 | 0.1791 | 0.1718 | 0.1755 | 0.1613 |
| 222.22 | 0.5172 | 0.5250 | 0.5211 | 0.5069 |
| 666.67 | 1.4860 | 1.4850 | 1.4855 | 1.4713 |
| 2000.00 | 3.3930 | 3.4200 | 3.4065 | 3.3923 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 27.3 | 173.6 | 525.6 | 39.3 | 185.7 | 604.2 |
| Standard Deviation | 2.1 | 11.6 | 25.5 | 3.1 | 13.1 | 37.2 |
| Coefficient of Variation (%) | 7.9 | 6.7 | 4.9 | 8.0 | 7.1 | 6.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human cIAP2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 102% to 119% with an overall mean recovery of 110%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-4.39 | 33.3 | 1.8 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of cIAP2 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
AIP 1
AIP1
API 2
API2
Apoptosis inhibitor 2
Baculoviral IAP repeat containing 3
Baculoviral IAP repeat containing protein 3
Baculoviral IAP repeat-containing protein 3
BIRC 3
BIRC3
展開