Human CD33 enzyme-linked immunoassay kit

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Catalog# EH0106

Human CD33 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

4.12~3000 pg/ml

製品概要

製品名

Human CD33 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.83 pg/ml (50 μl)；0.31 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0157	0.0173	0.0165
4.12	0.0251	0.0252	0.0252	0.0087
12.35	0.0497	0.0455	0.0476	0.0311
37.04	0.1108	0.1200	0.1154	0.0989
111.11	0.2884	0.2943	0.2914	0.2749
333.33	0.7946	0.8016	0.7981	0.7816
1000.00	2.2380	2.2760	2.2570	2.2405
3000.00	4.0429	4.0558	4.0494	4.0329

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	67.0	326.3	928.5	58.7	306.1	975.6
Standard Deviation	3.3	12.0	40.9	3.2	22.3	49.2
Coefficient of Variation (%)	5.0	3.7	4.4	5.4	7.3	5.0

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human CD33 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 96% to 107% with an overall mean recovery of103%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	1005.10-8398.28	100	3736.54

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P20138 Human

別名

CD 33

CD33

CD33 antigen (gp67)

CD33 antigen

CD33 molecule

CD33_HUMAN

FLJ00391

gp67

My9

Myeloid cell surface antigen CD33

展開

機能

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N‑terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system. This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasma tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system. Human Siglec-3 is alternatively known as myeloid cell surface antigen CD33 and GP67. Human Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, one Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. Siglec-3 expression is restricted to cells of myelomonocytic lineage. It binds sialic acid preferring alpha 2,3- linkage over alpha 2,6- linkage. Studies indicated that Siglec-3 recruits SHP-1 and SHP-2 to its ITIMs. When co-crosslinking with Fc gamma R1, Siglec-3 inhibits tyrosine phosphorylation and calcium mobilization, suggesting Siglec-3 can mediate inhibitory signals.

Human CD33 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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