Human Elafin/PI3 enzyme-linked immunoassay kit
Catalog# EH0236
Human Elafin/PI3 enzyme-linked immunoassay kit
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ELISA
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Human
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78.13~5000 pg/ml
製品概要
製品名
Human Elafin/PI3 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
3.86 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0106 | 0.0116 | 0.0111 | |
| 78.13 | 0.0346 | 0.0359 | 0.0353 | 0.0242 |
| 156.25 | 0.0793 | 0.0779 | 0.0786 | 0.0675 |
| 312.50 | 0.2053 | 0.2213 | 0.2133 | 0.2022 |
| 625.00 | 0.5769 | 0.5912 | 0.5841 | 0.5730 |
| 1250.00 | 1.3420 | 1.2700 | 1.3060 | 1.2949 |
| 2500.00 | 2.5920 | 2.5860 | 2.5890 | 2.5779 |
| 5000.00 | 3.5800 | 3.5990 | 3.5895 | 3.5784 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 129.1 | 645.0 | 1969.1 | 104.6 | 632.9 | 2056.2 |
| Standard Deviation | 8.5 | 46.2 | 150.3 | 4.7 | 34.1 | 115.4 |
| Coefficient of Variation (%) | 6.6 | 7.2 | 7.6 | 4.5 | 5.4 | 5.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Elafin/PI3 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 118% with an overall mean recovery of 102%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 501.95-1527 | 100 | 1000.09 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存