Human RAGE enzyme-linked immunoassay kit（one step）
Catalog# EHY0222
Human RAGE enzyme-linked immunoassay kit（one step）
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ELISA
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Human
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156.25~10000 pg/ml
製品概要
製品名
Human RAGE enzyme-linked immunoassay kit（one step）
製品タイプ
Elisa Kit
検出感度
21.39 pg/ml (50 μl)；65.37 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0068 | 0.0060 | 0.0064 | |
| 156.25 | 0.0178 | 0.0185 | 0.0182 | 0.0118 |
| 312.50 | 0.0346 | 0.0384 | 0.0365 | 0.0301 |
| 625.00 | 0.0884 | 0.0897 | 0.0891 | 0.0827 |
| 1250.00 | 0.2429 | 0.2499 | 0.2464 | 0.2400 |
| 2500.00 | 0.6810 | 0.6999 | 0.6905 | 0.6841 |
| 5000.00 | 1.8460 | 1.8980 | 1.8720 | 1.8656 |
| 10000.00 | 3.8120 | 3.8160 | 3.8140 | 3.8076 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 201.9 | 1011.4 | 3082.6 | 218.5 | 1013.6 | 3171.6 |
| Standard Deviation | 9.6 | 48.7 | 122.7 | 7.2 | 33.4 | 105.4 |
| Coefficient of Variation (%) | 4.8 | 4.8 | 4.0 | 3.3 | 3.3 | 3.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human RAGE into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 122% with an overall mean recovery of 102%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 720.92-9110.87 | 100 | 2431.31 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of RAGE in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
Advanced glycosylation end product-specific receptor
Ager
MGC2235
RAGE_HUMAN
Receptor for advanced glycosylation end products