Human CDCP1 enzyme-linked immunoassay kit
Catalog# EH0083
Human CDCP1 enzyme-linked immunoassay kit
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ELISA
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Human
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31.25~2000 pg/ml
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unconjugated
製品概要
製品名
Human CDCP1 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
1.41 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0241 | 0.0233 | 0.0237 | |
| 31.25 | 0.0716 | 0.0819 | 0.0768 | 0.0531 |
| 62.50 | 0.1091 | 0.1151 | 0.1121 | 0.0884 |
| 125.00 | 0.1978 | 0.2099 | 0.2039 | 0.1802 |
| 250.00 | 0.3730 | 0.3911 | 0.3821 | 0.3584 |
| 500.00 | 0.7124 | 0.7447 | 0.7286 | 0.7049 |
| 1000.00 | 1.3820 | 1.4660 | 1.4240 | 1.4003 |
| 2000.00 | 2.7060 | 2.8110 | 2.7585 | 2.7348 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 10.9 | 55.0 | 157.3 | 10.0 | 49.7 | 152.0 |
| Standard Deviation | 0.8 | 3.0 | 8.1 | 0.5 | 2.1 | 5.5 |
| Coefficient of Variation (%) | 7.1 | 5.4 | 5.1 | 5.4 | 4.2 | 3.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human CDCP1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 79% to 120% with an overall mean recovery of 108%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 96.09-307.20 | 100 | 196.05 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CDCP1 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
9030022E12Rik
AA409659
CD 318
CD_antigen=CD318
CD318
CD318 antigen
CDCP 1
Cdcp1
CDCP1_HUMAN
CUB domain containing protein 1
展開