Human CXADR/CAR enzyme-linked immunoassay kit(one step)
Catalog# EHY0262
Human CXADR/CAR enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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13.72~10000 pg/ml
製品概要
製品名
Human CXADR/CAR enzyme-linked immunoassay kit(one step)
製品タイプ
Elisa Kit
検出感度
2.64 pg/ml (50 μl)；3.77 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0061 | 0.0059 | 0.0060 | |
| 13.72 | 0.0100 | 0.0096 | 0.0098 | 0.0038 |
| 41.15 | 0.0194 | 0.0191 | 0.0193 | 0.0133 |
| 123.46 | 0.0491 | 0.0510 | 0.0501 | 0.0441 |
| 370.37 | 0.1454 | 0.1401 | 0.1428 | 0.1368 |
| 1111.11 | 0.4314 | 0.4186 | 0.4250 | 0.4190 |
| 3333.33 | 1.1710 | 1.1640 | 1.1675 | 1.1615 |
| 10000.00 | 2.9580 | 2.9080 | 2.9330 | 2.9270 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 248.5 | 1141.3 | 3266.2 | 246.9 | 1170.4 | 3427.4 |
| Standard Deviation | 7.2 | 26.1 | 129.8 | 7.3 | 47.9 | 102.1 |
| Coefficient of Variation (%) | 2.9 | 2.3 | 4.0 | 2.8 | 3.0 | 3.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of Human CXADR/CAR into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 101% with an overall mean recovery of 95%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 18.04-219.66 | 100 | 82.94 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存