Human FOLR1 enzyme-linked immunoassay kit
Catalog# EH0137
Human FOLR1 enzyme-linked immunoassay kit
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ELISA
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Human
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46.88~3000 pg/ml
製品概要
製品名
Human FOLR1 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
2.40 pg/ml (50 μl)；3.97 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0158 | 0.0153 | 0.0156 | |
| 46.88 | 0.1271 | 0.1112 | 0.1192 | 0.1036 |
| 93.75 | 0.2292 | 0.2275 | 0.2284 | 0.2128 |
| 187.50 | 0.4572 | 0.4467 | 0.4520 | 0.4364 |
| 375.00 | 0.9516 | 0.9921 | 0.9719 | 0.9563 |
| 750.00 | 1.9200 | 2.0350 | 1.9775 | 1.9620 |
| 1500.00 | 3.9130 | 3.9100 | 3.9115 | 3.8960 |
| 3000.00 | 4.5199 | 4.5427 | 4.5313 | 4.5158 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 50.8 | 260.6 | 771.5 | 49.9 | 237.4 | 720.3 |
| Standard Deviation | 3.5 | 14.6 | 48.5 | 2.7 | 13.0 | 38.8 |
| Coefficient of Variation (%) | 6.8 | 5.6 | 6.3 | 5.3 | 5.5 | 5.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human FOLR1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 76% to 120% with an overall mean recovery of 101%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 104.68-266.14 | 100 | 178.81 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of FOLR1 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
adult
Adult folate binding protein
Adult folate-binding protein
FBP
Folate Binding Protein
Folate Receptor 1 Adult
Folate receptor 1
Folate Receptor 1 Precursor
Folate receptor adult
Folate receptor alpha
展開