Human IL-1 alpha enzyme-linked immunoassay kit

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Catalog# EH0243

Human IL-1 alpha enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

2.74~2000 pg/ml

製品概要

製品名

Human IL-1 alpha enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

1.37 pg/ml (50 μl)；6.71 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0080	0.0077	0.0079
2.74	0.0134	0.0135	0.0135	0.0056
8.23	0.0254	0.0229	0.0242	0.0163
24.69	0.0543	0.0559	0.0551	0.0473
74.07	0.1547	0.1531	0.1539	0.1461
222.22	0.4364	0.4292	0.4328	0.4250
666.67	1.1850	1.2130	1.1990	1.1912
2000.00	2.9320	2.9920	2.9620	2.9542

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	38.1	186.6	595.9	40.2	194.7	593.9
Standard Deviation	1.9	8.6	25.6	1.7	8.6	21.6
Coefficient of Variation (%)	5.1	4.6	4.3	4.2	4.4	3.6

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human IL-1 alpha into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 100% to 120% with an overall mean recovery of 115%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	n.d.-28.38	73.3	7.14

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P01583 Human

機能

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia). IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

Human IL-1 alpha enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

機能

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