Human IFN-γ Super Sensitivity enzyme-linked immunoassay kit

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Catalog# EHSS0039

Human IFN-γ Super Sensitivity enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

0.14~100 pg/ml

製品概要

製品名

Human IFN-γ Super Sensitivity enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.02 pg/ml (50 μl)；0.17 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0382	0.0356	0.0369
0.14	0.0482	0.0460	0.0471	0.0102
0.41	0.0593	0.0621	0.0607	0.0238
1.23	0.1022	0.1060	0.1041	0.0672
3.70	0.2399	0.2478	0.2439	0.2070
11.11	0.6023	0.6231	0.6127	0.5758
33.33	1.8669	1.7010	1.7840	1.7471
100.00	4.0051	4.0133	4.0092	3.9723

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	1.6	10.0	29.9	1.8	9.7	29.6
Standard Deviation	0.1	0.4	1.4	0.1	0.5	1.4
Coefficient of Variation (%)	6.5	4.5	4.8	5.6	4.7	4.6

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human IFN gamma into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 89% to 113% with an overall mean recovery of 105%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	0.50-65.20	100	5.94

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IFN gamma in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P01579 Human

別名

IFG

IFI

IFN gamma

IFN, immune

IFN-gamma

IFNG

IFNG_HUMAN

Immune interferon

Interferon gamma

Sensitive

機能

Interferon-gamma (IFN-gamma), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59%‑64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37%‑43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Human IFN-γ Super Sensitivity enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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