Human Complement factor H enzyme-linked immunoassay kit
Catalog# EH0246
Human Complement factor H enzyme-linked immunoassay kit
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ELISA
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Human
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13.72~10000 pg/ml
製品概要
製品名
Human Complement factor H enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
1.37 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0249 | 0.0241 | 0.0245 | |
| 13.72 | 0.0447 | 0.0429 | 0.0438 | 0.0193 |
| 41.15 | 0.0872 | 0.0895 | 0.0884 | 0.0639 |
| 123.46 | 0.2038 | 0.2194 | 0.2116 | 0.1871 |
| 370.37 | 0.5905 | 0.5950 | 0.5928 | 0.5683 |
| 1111.11 | 1.4090 | 1.4310 | 1.4200 | 1.3955 |
| 3333.33 | 2.5530 | 2.5420 | 2.5475 | 2.5230 |
| 10000.00 | 3.3510 | 3.3810 | 3.3660 | 3.3415 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 180.0 | 919.1 | 2801.6 | 269.2 | 1214.9 | 3867.2 |
| Standard Deviation | 9.5 | 45.6 | 146.1 | 10.9 | 52.4 | 209.7 |
| Coefficient of Variation (%) | 5.3 | 5.0 | 5.2 | 4.0 | 4.3 | 5.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Complement factor H into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 82% to 96% with an overall mean recovery of 90%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 153.70-616.01 | 100 | 377.60 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存