Human WNT7A enzyme-linked immunoassay kit
Catalog# EH0312
Human WNT7A enzyme-linked immunoassay kit
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ELISA
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Human
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40.96 ~10000 pg/ml
製品概要
製品名
Human WNT7A enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
3.62 pg/ml (50 μl)；7.94 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0164 | 0.0152 | 0.0158 | |
| 40.96 | 0.0351 | 0.0332 | 0.0342 | 0.0184 |
| 102.40 | 0.0619 | 0.0631 | 0.0625 | 0.0467 |
| 256.00 | 0.1375 | 0.1384 | 0.1380 | 0.1222 |
| 640.00 | 0.3264 | 0.3251 | 0.3258 | 0.3100 |
| 1600.00 | 0.7644 | 0.7827 | 0.7736 | 0.7578 |
| 4000.00 | 1.7650 | 1.7600 | 1.7625 | 1.7467 |
| 10000.00 | 3.6480 | 3.6180 | 3.6330 | 3.6172 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 138.8 | 772.3 | 2512.3 | 226.3 | 1112.0 | 3465.9 |
| Standard Deviation | 10.5 | 40.6 | 169.6 | 14.9 | 20.6 | 123.0 |
| Coefficient of Variation (%) | 7.6 | 5.3 | 6.8 | 6.6 | 1.9 | 3.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human WNT7A into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 109% with an overall mean recovery of 94%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-1.92 | 31.25 | 1.26 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human WNT7A in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存