Human SAA1 enzyme-linked immunoassay kit
Catalog# EH0180
Human SAA1 enzyme-linked immunoassay kit
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ELISA
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Human
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1.56~100 ng/ml
製品概要
製品名
Human SAA1 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.30pg/ml (50 μl)；1.56 pg/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0226 | 0.0227 | 0.0227 | |
| 1.56 | 0.0334 | 0.0370 | 0.0352 | 0.0126 |
| 3.13 | 0.0579 | 0.0619 | 0.0599 | 0.0373 |
| 6.25 | 0.1442 | 0.1511 | 0.1477 | 0.1250 |
| 12.50 | 0.3880 | 0.4018 | 0.3949 | 0.3723 |
| 25.00 | 1.2466 | 1.2545 | 1.2506 | 1.2279 |
| 50.00 | 3.1485 | 3.0604 | 3.1045 | 3.0818 |
| 100.00 | 4.6630 | 4.7992 | 4.7311 | 4.7085 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 2.4 | 12.0 | 38.0 | 2.5 | 11.4 | 35.1 |
| Standard Deviation | 0.1 | 0.4 | 1.3 | 0.1 | 0.5 | 1.3 |
| Coefficient of Variation (%) | 5.6 | 3.0 | 3.5 | 2.9 | 4.1 | 3.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human SAA1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 117% with an overall mean recovery of 100%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 24.57-213.30 | 100 | 118.34 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of SAA1 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
PIG4
SAA
SAA1
SAA2
Serum amyloid A 2 protein
Serum amyloid A protein
Serum amyloid A1
Serum amyloid A2
TP53I4
Tumor protein p53 inducible protein 4