Human CD28 enzyme-linked immunoassay kit
Catalog# EH0183
Human CD28 enzyme-linked immunoassay kit
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ELISA
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Human
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156.25~10000 pg/ml
製品概要
製品名
Human CD28 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
1.41 pg/ml (50 μl)；57.28 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0102 | 0.0092 | 0.0097 | |
| 156.25 | 0.0309 | 0.0313 | 0.0311 | 0.0214 |
| 312.50 | 0.0496 | 0.0578 | 0.0537 | 0.0440 |
| 625.00 | 0.0966 | 0.1092 | 0.1029 | 0.0932 |
| 1250.00 | 0.2040 | 0.2336 | 0.2188 | 0.2091 |
| 2500.00 | 0.5139 | 0.5597 | 0.5368 | 0.5271 |
| 5000.00 | 1.3250 | 1.5090 | 1.4170 | 1.4073 |
| 10000.00 | 3.3340 | 3.5900 | 3.4620 | 3.4523 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 203.4 | 1052.0 | 3276.1 | 166.4 | 957.1 | 3088.7 |
| Standard Deviation | 11.5 | 37.4 | 176.2 | 3.5 | 29.7 | 91.4 |
| Coefficient of Variation (%) | 5.6 | 3.6 | 5.4 | 2.1 | 3.1 | 3.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human CD28 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 113% with an overall mean recovery of 103%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 3.32-816.93 | 100 | 104.41 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD28 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
CD 28
CD28
CD28 antigen
CD28 molecule
CD28_HUMAN
MGC138290
T cell antigen CD28
T cell specific surface glycoprotein
T cell specific surface glycoprotein CD28
T-cell-specific surfac