Human Leptin Receptor enzyme-linked immunoassay kit

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Catalog# EH0187

Human Leptin Receptor enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

13.72~10000 pg/ml

製品概要

製品名

Human Leptin Receptor enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.90 pg/ml (50 μl)；5.07 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0088	0.0092	0.0090
13.72	0.0208	0.0210	0.0209	0.0119
41.15	0.0539	0.0514	0.0527	0.0437
123.46	0.1480	0.1444	0.1462	0.1372
370.37	0.4646	0.4120	0.4383	0.4293
1111.11	1.1680	1.1840	1.1760	1.1670
3333.33	2.5250	2.4640	2.4945	2.4855
10000.00	4.1075	4.1126	4.1101	4.1011

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	272.7	915.8	3089.71	303.1	985.9	3109.3
Standard Deviation	13.9	42.7	174.51	11.4	46.1	189.2
Coefficient of Variation (%)	5.1	4.7	5.6	3.8	4.7	6.1

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human Leptin Receptor into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 106% with an overall mean recovery of 93%.

サンプル値

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	24.77-61.58	100	37.42

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Leptin Receptor in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P48357 Human

別名

CD 295

CD295

CD295 antigen

HuB219

LEP R

LEP-R

LEPR

LEPR_HUMAN

展開

機能

Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-RL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-Rs) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript lacking a transmembrane domain and potentially encoding a soluble form of the receptor has also been described. Circulating soluble OB-R, complexed to leptin, has been detected in mouse serum. Serum soluble OB-R levels have been shown to increase during pregnancy. OB-RL transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-Rs transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney and primitive hematopoietic cell populations. OB-R has recently been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype. Mouse OB-R long form encodes a 1162 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 817 aa residue extracellular domain, a 21 aa residue transmembrane domain, and a 302 aa residue cytoplasmic domain. The extracellular domain of OB-R contains two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant murine soluble OB-R has been shown to bind leptin with high affinity and is a potent leptin antagonist.

Human Leptin Receptor enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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