Human Tau//MAPT enzyme-linked immunoassay kit

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Catalog# EH0295

Human Tau//MAPT enzyme-linked immunoassay kit

Application

sELISA

Reactivity

Human

Standard curve range

20.48 ~5000 pg/ml

製品概要

製品名

Human Tau//MAPT enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

3.47pg/ml (50 μl)；6.37 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0292	0.0295	0.0294
20.48	0.0506	0.0537	0.0522	0.0228
51.20	0.0794	0.0868	0.0831	0.0538
128.00	0.1494	0.1625	0.1560	0.1266
320.00	0.2918	0.3269	0.3094	0.2800
800.00	0.6706	0.7848	0.7277	0.6984
2000.00	1.5620	1.7330	1.6475	1.6182
5000.00	3.5600	3.6420	3.6010	3.5717

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	74.2	409.6	1309.9	92.4	497.6	1581.1
Standard Deviation	5.5	19.3	63.4	4.4	24.1	36.4
Coefficient of Variation (%)	7.5	4.7	4.8	4.7	4.8	2.3

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human Tau/MAPT into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 107% with an overall mean recovery of 98%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	n.d.-45.74	43.75	13.76

Serum/Plasma– Thirty samples from apparently healthy volunteers serum were evaluated for the presence of human Tau/MAPT in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P10636 Human

機能

Tau is a relatively low molecular weight member of the microtubule associated protein or MAP family. Most of these proteins were discovered since microtubules can be polymerized in cell homogenates and pelleted out by centrifugation, typically taking MAP proteins with them. This early work showed that the Tau protein facilitated the polymerization of microtubules, and was therefore named after the Greek letter tau, since it promoted tubule formation. Tau is heavily concentrated in axons of neurons, but may also be found in dendrites and in some non-neuronal cells. Much interest has focused on Tau as it is a major component of the neurofibrillary tangles of Alzheimer's disease. Tau in neurofibrillary tangles is typically heavily and aberrantly phosphorylated, and it is believed that phosphorylation may be involved in tangle formation. In addition, numerous different point mutations in the Tau gene are causative of Frontotemporal dementia with Parkinsonism linked to chromosome 17 (FTDP-17). There is one mammalian Tau gene which produces at least 9 different proteins by alternate transcription. In the central nervous system 6 isoforms predominant which either include or do not include three short exon coded inserts. These proteins range in size from 352-441 amino acids and run on SDS-PAGE gels as multiple bands ranging from 48-67 kDa. In peripheral nervous system a form called "big tau" predominates, another alternate transcript which includes a 254 amino acid insert. This form of Tau is found in small amounts in the brain also, in cranial nerve motor nuclei and sensory processes of most sensory ganglia, and runs on SDS-PAGE with an apparent molecular weight of 100 kDa. Each Tau protein contains 3 or 4 copies of an 18 amino acid peptide which are responsible for binding to the microtubules and are similar to those found in MAP2 and other members of the MAP family. Tau is a highly charged acidic protein with few hydrophobic residues which belongs to the family of "intrinsically unstructured proteins". As with GAP43, MARCKS and several other similar proteins, Tau isoforms run on SDS-PAGE much more slowly than expected from their actual molecular weight. This antibody was made against a recombinant construct expressed in and purified from E. coli and which corresponded to the shortest version of the various Tau proteins, so this antibody is expected to bind to all Tau isoforms.

Human Tau//MAPT enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

機能

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