Human Resistin enzyme-linked immunoassay kit
Catalog# EH0206
Human Resistin enzyme-linked immunoassay kit
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ELISA
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Human
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13.72~10000 pg/ml
製品概要
製品名
Human Resistin enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.33 pg/ml (50 μl)；0.48 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0118 | 0.0113 | 0.0116 | |
| 13.72 | 0.0165 | 0.0175 | 0.0170 | 0.0055 |
| 41.15 | 0.0270 | 0.0278 | 0.0274 | 0.0159 |
| 123.46 | 0.0605 | 0.0604 | 0.0605 | 0.0489 |
| 370.37 | 0.1887 | 0.1819 | 0.1853 | 0.1738 |
| 1111.11 | 0.5823 | 0.5926 | 0.5875 | 0.5759 |
| 3333.33 | 1.9340 | 1.8680 | 1.9010 | 1.8895 |
| 10000.00 | 4.2792 | 4.3434 | 4.3113 | 4.2998 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 173.5 | 899.9 | 3107.1 | 179.8 | 932.5 | 3171.0 |
| Standard Deviation | 6.8 | 32.8 | 125.6 | 3.0 | 17.6 | 149.7 |
| Coefficient of Variation (%) | 3.9 | 3.6 | 4.0 | 1.7 | 1.9 | 4.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Resistin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 116% with an overall mean recovery of 103%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.81-34.93 | 100 | 18.17 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Resistin in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
Adipose tissue specific secretory factor
Adipose tissue-specific secretory factor
ADSF
C/EBP epsilon regulated myeloid specific secreted cysteine rich protein
C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1
C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein
CG5403
Cysteine rich secreted protein A12 alpha like 2
Cysteine rich secreted protein FIZZ3
Cysteine-rich secreted protein A12-alpha-like 2
展開