Human IGFBP6 enzyme-linked immunoassay kit(one step)
Catalog# EHY0336
Human IGFBP6 enzyme-linked immunoassay kit(one step)
-
sELISA
-
Human
-
0.14 ~100 ng/ml
製品概要
製品名
Human IGFBP6 enzyme-linked immunoassay kit(one step)
製品タイプ
Elisa Kit
検出感度
0.01ng/ml (50 μl)；0.06ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0129 | 0.0134 | 0.0132 | |
| 0.14 | 0.0244 | 0.0238 | 0.0241 | 0.0110 |
| 0.41 | 0.0473 | 0.0467 | 0.0470 | 0.0339 |
| 1.23 | 0.1208 | 0.1193 | 0.1201 | 0.1069 |
| 3.70 | 0.3341 | 0.3367 | 0.3354 | 0.3223 |
| 11.11 | 1.0190 | 1.0020 | 1.0105 | 0.9974 |
| 33.33 | 2.5820 | 2.5930 | 2.5875 | 2.5744 |
| 100.00 | 4.0854 | 4.1603 | 4.1229 | 4.1097 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.8 | 9.1 | 32.6 | 3.1 | 11.9 | 28.5 |
| Standard Deviation | 0.1 | 0.3 | 1.1 | 0.1 | 0.4 | 0.5 |
| Coefficient of Variation (%) | 3.1 | 2.9 | 3.5 | 3.0 | 3.5 | 1.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of Human IGFBP6 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 119% with an overall mean recovery of 105%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 50.24-536.72 | 100 | 261.23 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Human IGFBP6 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
1
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
Insulin-like growth factor-binding protein 6
IBP-6
IGF-binding protein 6
IGFBP-6
IGFBP6
IBP6